3.4.22.B14	-	
3.4.22.B14	cloned into plasmids under T7 promoter control and cotransfected into HeLa-MHVR cells infected with recombinant vaccinia virus	
3.4.22.B14	expressed in Escherichia coli	
3.4.22.B14	expressed in Escherichia coli Rosetta (DE3)pLysS cells	
3.4.22.B14	expression in Escherichia coli	
3.4.22.B14	expression in Escherichia coli by using a T7 RNA polymerase promoter system or as a maltose-binding protein fusion protein. With both overexpression systems the recombinant PLP-1 exhibits trans cleavage activity. When PLP-1 is expressed as a polypeptide that includes additional viral sequences at the carboxyl terminus of the predicted PLP-1 domain, a fivefold increase in proteolytic activity is observed