3.4.22.53	-	643974, 644011, 685818	
3.4.22.53	a 629 bp fragment is cloned into the EcoRV site of pBluescript II KS+ vector	643972	
3.4.22.53	cDNA fragments corresponding to the domains with E-F hand structures in the large and small subunits are inserted into an expression vector pIC18 or pUC8. The resulting plasmids are used to transform Escherichia coli and isopropyl-1-thio-beta-D-galactoside-inducible expression is induced	643971	
3.4.22.53	cloning of the cDNA for the large subunit	644009	
3.4.22.53	coexpression from large-subunit and small-subunit plasmids in Escherichia coli strain BL21(DE3)	643968	
3.4.22.53	development of an adenoviral vector harboring calpain-2 siRNA expression unit in which sense and anti-sense strands composing the siRNA duplex are connected by a loop and transcribed into a siRNA in porcine pulmonary artery endothelial cells. The adenoviral vector harboring calpain-2 siRNA expression unit is a valuable tool to study the biology of calpains	688945	
3.4.22.53	expressed in Escherichia coli	732375	
3.4.22.53	expression of mutated calpain 2 C105A is driven in lens by coupling the mutated gene to the betaB1-crystallin promoter	667378	
3.4.22.53	functional analysis of the upstream region of the gene for the large subunit by means of transient expression assay on HeLa cells using chloramphenicol transferase constructs identifies four negative regulatory regions tandemly reiterated just upstream of the promoter region, P1 and P2	644010	
3.4.22.53	isolation of cDNA clone for the 80000 Da subunit	643970