3.4.21.B42 expressed in Neuro2a cells 679747 3.4.21.B42 gene KLK11, identical to gene TLSP or PRSS20, splicing variant 1, prostate-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection 647365 3.4.21.B42 gene KLK11, splicing variant 2, brain-type isozyme, DNA sequence determination and analysis, transient expression of Myc-His-tagged enzyme in COS-7 cells, expression as fusion protein with trypsinogen signal peptide followed by a His-tag in insect cells via baculovirus infection 647365 3.4.21.B42 molecular cloning of four novel splice variants of the human KLK11 gene, discovered by combining 3' rapid amplification of cDNA ends (3' RACE), NGS technology, advanced bioinformatic analysis, and Sanger sequencing 752890