3.4.16.6	-	647232, 647235	
3.4.16.6	a truncated form of Kex-1, Kex-1-C611, is constructed comprising the amino acid residues from 104-611 and lacks the transmembrane domain	701056	
3.4.16.6	a truncated form of KexA (amino acid residues 1 to 526 of KexA), which lack the potential transmembrane region and the succeeding C-terminal sequence (amino acid residues 527 to 625 of KexA) is expressed by using a protein expression system with the pIECS3 vector and Aspergillus nidulans	731176	
3.4.16.6	individual domains of the enzyme are expressed in insect Sf9 cells using the baculovirus expression system. Medium from domain 1B-expressing cells and domain 2-expressing cells shows substantial enzymatic activity, whereas medium from domain 1A-expressing cells is not different from cells infected with wild-type virus. The individual domains 1A, 1B, and 2 are expressed in baculovirus under the polyhedrin promoter and with the signal peptide of the rat enzyme	647241	
3.4.16.6	into the yeast shuttle vector pVT100-ZZ	698771	
3.4.16.6	the transthyretin-like domain belonging to the first catalytic domain of human metallocarboxypeptidase D (residues 386-460, h-TTL), is cloned into the pET-22B vector to encode a C-terminal hexahistidine fusion protein. Expression carried out in Escherichia coli	732160