2.7.4.25	expressed in Escherichia coli BL21(DE3) cells	
2.7.4.25	expression of wild-type and mutant enzymes	
2.7.4.25	hanging-drop method in 50 mM Tris-HCl buffer, pH 7.4, 20°C, with ammonium sulfate as a precipitant	
2.7.4.25	overexpression in Escherichia coli	
2.7.4.25	overexpression in Escherichia coli to produce CTP