2.3.1.95 - 689996, 736807, 758403 2.3.1.95 co-expression of AhSTS3 with Sorghum bicolor O-methyltransferase OMT3, EC 2.1.1.240, in Arabidopsis thaliana and Nicotiana tabacum using the CaMV 35S promoter and the Agrobacterium tumefaciens transfection method, quantitative realtime RT-PCR expression analysis in transgenic leaf samples, phenotypic analysis of pCRO1 transformants, overview. Subcloning in Escherichia coli. Binary vector construction 720646 2.3.1.95 expression in Arabidopsis thaliana 757328 2.3.1.95 expression in Escherichia coli 487729, 487730, 689646 2.3.1.95 expression in Escherichia coli and Oryza sativa 737086 2.3.1.95 expression of fusion enzymes in Escherichia coli 689646 2.3.1.95 expression of wild-type, P375G and G374L mutant enzyme in Escherichia coli 487726 2.3.1.95 gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis 718636 2.3.1.95 Humulus lupulus plants of the Tettnang variety are transformed with a gene encoding for STS from grapevine. Under the control of the constitutive 35S cauliflower mosaic virus promoter, expression of the transgene results in accumulation of resveratrol and high levels of its glycosylated derivatives in leaves and inflorescences. Piceid, the predominant derivative, reaches a concentration of up to 560 microg/g of fresh weight in hop cones, whereas no stilbenes are detected in nontransformed controls (wild type). In Humulus lupulus constitutive expression of sts interferes neither with plant development nor with the biosynthesis of secondary metabolites relevant for the brewing industry. Since resveratrol is a well-known phytoalexin and antioxidant, transgenic Humulus lupulus plants could display enhanced pathogen resistance against microbial pathogens, exhibit new beneficial properties for health, and open new venues for metabolic engineering 687242 2.3.1.95 PcRS gene , recombinant expression in Arabidopsis thaliana under the control of CaMV 35S promoter using the Agrobacterium tumefaciens LBA 4404 transfection method 720665