2.3.1.191	-	700935, 735384, 736880	
2.3.1.191	by complementation of a temperature-sensitive Escherichia coli lpxD mutant, a meningococcal chromosomal fragment is cloned that carries the lpxD homologue	698088	
2.3.1.191	expression in Escherichia coli BL21(DE3)	755699	
2.3.1.191	gene AtlpxD, two AtLpxD isozymes, AtLxpD1 and AtLpxD2, are encoded on chromosome 4, DNA and amino acid sequence determination and analysis, expression as GFP-tagged protein in Arabidopsis thaliana	720913	
2.3.1.191	gene lpxD, expression as His6-tagged protein in Escherichia coli strain BL21(DE3)	718571	
2.3.1.191	gene lpxD, expression of N-terminally His6-tagged LpxD in Escherichia coli strain BL21 (DE3)	718513	
2.3.1.191	genes lpxD1 and lpxD2, DNA and amino acid sequence determination and analysis, phyylogenetic analysis, the lpxD1gene lies within an essential four-gene operon, lpxD1/fabZ/lpxA/lpxB, while the second gene lpxD2 as a single gene is predicted to be regulated by its own promoter	720944	
2.3.1.191	lpxA (lpxAPg) and lpxDPg are cloned and expressed in Escherichia coli strains in which the homologous gene is mutated. Lipid A from strains expressing either of the Porphyromonas gingivalis transferases contains 16-carbon hydroxy fatty acids in addition to the normal Escherichia coli 14-carbon hydroxy fatty acids, demonstrating that these acyltransferases display a relaxed acyl chain length specificity	698587	
2.3.1.191	LpxD protein modified with an N-terminal His6 tag followed by a one glycine residue linker and the P2A substitution, is constructed and transformed into Escherichia coli Rosetta (DE3)/pLysS	696356	
2.3.1.191	overexpression in Escherichia coli	696264