2.3.1.135 - 674832, 685367 2.3.1.135 DNA sequence determination and analysis of the full length gene including the 3'- and 5'-ends, genetic organization analysis, at least 2 splicing variants, the second of which lacks a 103 nt polynucleotide in the 5'-UTR of the full length transcript 658821 2.3.1.135 expression in HEK-293 cells 638617 2.3.1.135 expression in HEK-293T cells 638617, 638618, 638619, 638620, 638621, 638624 2.3.1.135 expression in NIH-3T3 cell 756046 2.3.1.135 expression of a truncated enzyme form lacking the transmembrane N- and C-termini in Escherichia coli as His-tagged protein, which is insoluble in absence of detergents 658001 2.3.1.135 expression of His-tagged truncated wild-type enzyme and His-tagged mutant enzymes in Escherichia coli strain BL21(DE3) 658075 2.3.1.135 expression of wild-type and/or mutant enzymes inducibly in HEK-293 cells, transiently in COS-7 cells, and in Escherichia coli strain BL21(DE3) cells 674832 2.3.1.135 gene lrat is localized on chromosome 4 at locus 4q31.2 719417 2.3.1.135 gene Lrat, vector cloning for construction of knockout mutants 659309