2.1.1.294 gene warA, quantitative RT-PCR expression analysis, recombinant expression of N-terminally His- or GFP-tagged enzyme, recombinat expression of GST-His-tagged wild-type and mutant enzymes in Escherichia coli strain B834(DE3) 757781 2.1.1.294 overexpression of WbdD decreases O-polysaccharide chain length. O-polysaccharide chain length is not reduced by overexpressing the heterologous plasmid encoded WbdD proteins. The WbdD proteins are therefore specific for a given serotype 722640