2.1.1.282 gene trm5a, recombinant expression of wild-type and mutant enzymes 758348 2.1.1.282 gene trm5a, sequence comparisons, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 758376 2.1.1.282 hexahistidine-tagged enzyme is expressed in Saccharomyces cerevisiae 722112 2.1.1.282 recombinant expression of His-tagged enzyme 758324 2.1.1.282 recombinant expression of His-tagged wild-type and mutant enzymes 758324