1.14.14.3 - 348545, 348548, 348555, 348573, 765786 1.14.14.3 amplified from the pJHD500 plasmid and ligated into a pET21b vector, expressed from pZCH2 in an Escherichia coli BL21 (lambdaDE3) cell line after growth to an OD600 of 0.5 702318 1.14.14.3 coexpression of luciferase and cytochrome P-450 348602 1.14.14.3 expressed in Escherichia coli BL21(DE3) and HEK-293T cells 728017 1.14.14.3 expressed in Escherichia coli BL21(DE3) cells 728822 1.14.14.3 expressed in Escherichia coli from pJHD500, ligated into a pET21b vector. Luciferase subcloned from pZCH2 into a pASKIBA-3c vector with the restriction sites XbaI and XhoI. The resulting luciferase containing a strep-II tag on the C terminus of the beta-subunit (pZCB4) expressed 698992 1.14.14.3 expressed in Escherichia coli JM109 (native enzyme) and BL21(DE3) cells (luciferase-mOrange fusion enzyme) 728435 1.14.14.3 expressed in NIH-3T3 cells 728228 1.14.14.3 expressed in Pseudomonas putida mt-2 726764 1.14.14.3 expression analysis, cloning into expression vector pPL2lux useable as a reporter system based on the luciferase activity of the enzyme, overview 671457