1.14.13.83 Brucella melitensis cobG is PCR-amplified, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside 1.14.13.83 expressed in Escherichia coli BL21DE3 1.14.13.83 gene cobG heterologously expressed in Escherichia coli 1.14.13.83 genetic and sequence analysis 1.14.13.83 overexpressed in recombinant strains of Escherichia coli 1.14.13.83 Pseudomonas denitrificans cobG is PCR-amplified in plasmid pCR427 and cloned into pET14b, overexpression of recombinant enzyme in Escherichia coli BL21 (DE3) inducible with isopropyl-1-thio-beta-D-galactopyranoside