1.13.11.6 - 439379 1.13.11.6 expression in Escherichia coli 439377, 649382 1.13.11.6 expression in human embryonic kidney HEK-293 439371, 439375 1.13.11.6 recombinant expression of His6-MBP-tagged enzyme in Escherichia coli 741516 1.13.11.6 Y3HAO protein is subcloned into the pET-28a expression vector from extracted Saccharomyces cerevisiae genomic DNA, and the Y3HAO protein is highly expressed as a soluble protein in Escherichia coli strain BL21(DE3) with a six-residueHis tag attached to its N-terminus 677020