7.4.2.6 - 7.4.2.6 cloning and gene organization of the bldK locus, overview 7.4.2.6 cloning in Escherichia coli JM109, PepT1 translation in Xenopus oocytes 7.4.2.6 DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression using the pAN701 plasmid 7.4.2.6 expressed in Escherichia coli BL21(DE3) cells 7.4.2.6 expressed in Lactococcus lactis strain NZ9000 7.4.2.6 expressed in lung, liver and spleen of mice 7.4.2.6 expression of Arabidopsis thaliana AtOPT6 in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae 7.4.2.6 expression of C-terminal His6-tagged OppA, OppA I602C, and OppBCDF His6-tag on C-terminus of OppC in Lactococcus lactis 7.4.2.6 four opp operons, opp-1, opp-2, opp-3, and opp-4, plus an opp-5A gene encoding a putative peptide-binding protein, DNA and amino acid sequence determination and analysis, opp-1, opp-2, opp-3, and opp-4 operons are polycistronic and opp-5A is monocistronic, transcriptional profiles and sequence comparison. Gene opp-3, which encodes proteins most similar to known peptide transport proteins, displays the highest expression level, overview 7.4.2.6 gene appA, expression of soluble form of AppA fused to maltose-binding protein in Escherichia coli strain B834 periplasm 7.4.2.6 gene oppA, DNA and amino acid sequence determination and analysis, expression of the wild-type enzyme in Escherichia coli strain BL21(DE3) 7.4.2.6 gene oppA, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as mainly insoluble protein 7.4.2.6 library construction and subcloning in Escherichia coli strains XL-1 Blue and SM10, opp gene cluster encoding five isozymes OppA, OppB, OppC, OppD, and OppF, DNA and amino acid sequence determination and analysis 7.4.2.6 library construction and subcloning in Escherichia coli strains XL-1 Blue and SM10, opp gene cluster encoding five isozymes OppA, OppB, OppC, OppD, and OppF, DNA and amino acid sequence determination and analysis, expression in Vibrio fluvialis and Escherichia coli 7.4.2.6 operon of genes oppA1, oppA2, oppB, oppC, oppD, oppF 7.4.2.6 opp, genome-wide transcriptional profiling 7.4.2.6 optimization of AtOPT6 expression in oocytes by swapping the 5'-untranslated region of AtOPT6 with the 5'-UTR of ScOPT1 from Saccharomyces cerevisiae 7.4.2.6 PepT1 expression as sense and antisense construct 7.4.2.6 the expression of the OppA proteins is individually regulated, alternative sigma factors, RpoS and RpoN, regulate the expression of oppA5 but not that of other oppA genes, while BosR/Fur homologue interacts with the oppA4 promoter and another candidate transcription factor, EbfC, interacts with the oppA5 promoter, overview 7.4.2.6 the two genes oppA1 and oppA2 encoding oligopeptide permeases are located in the clavulinic acid cluster, DNA and amino acid sequence determination and analysis