6.3.4.2	-	
6.3.4.2	a carboxyl-terminal FLAG sequence is introduced after insertion of human CTPS1 into pCDNA 4 myc/his, expression by transient transfection of human embryonic kidney (HEK) 293 cells	
6.3.4.2	cDNA encoding the synthetase domain of human CTPS (isoform 1) is subcloned into the pNIC-Bsa4 vector, the resulting construct codes for a protein with an N-terminal hexahistidine tag with an integrated TEV protease-cleavage site, the entire plasmid is transformed in Escherichia coli BL-21	
6.3.4.2	cloned in Escherichia coli as a histidine-tagged fusion protein	
6.3.4.2	cloned in Escherichia coli BL-21 as an N-terminal 6xHis-tag fusion protein	
6.3.4.2	expressed in Escherichia coli BL21(DE3) cells	
6.3.4.2	expression in a ura7ura8 double mutant that lacks CTP synthetase activity	
6.3.4.2	expression in Escherichia coli	
6.3.4.2	expression of the human type II CTP synthetase gene in the CTP synthetase-deficient mutant Escherichia coli JF618 completely abolishes the need for exogenous cytidine for their survival	
6.3.4.2	expression of the mutant enzyme D149E in Escherichia coli	
6.3.4.2	expression of wild-type and mutant enzymes in HEK-293 cells, co-expression with GST-tagged Xenopus laevis peptidyl prolyl isomerase Pin1	
6.3.4.2	functional expression of human CTPS1 and CTPS2 genes that encode CTP synthetase enzymes in enzyme-deficient Saccharomyces cerevisiae ura7D/ura8D double mutant, complementation	
6.3.4.2	genes ura7D and ura8D, DNA and amino acid sequence determination and analysis, expression analysis	
6.3.4.2	human CTP synthetase 1 is expressed and purified from a Saccharomyces cerevisiae ura7delta ura8delta double mutant that lacks CTP synthetase activity	
6.3.4.2	overexpression as a hexahistidine-tagged form	
6.3.4.2	protein is expressed as a His6-tagged wild type human CTP synthetase 1 fusion protein in Escherichia coli	
6.3.4.2	protein is expressed as a His6-tagged wild type human CTP synthetase 1 fusion protein in Saccharomyces cerevisiae	
6.3.4.2	synthetase-His6-TEV protease site-tagged Trypanosoma brucei CTP synthetase is expressed in BL21(DE3) pLysS bacteria	
6.3.4.2	wild-type and L109A recombinant enzyme are expressed in Escherichia coli BL21(DE3)