5.1.3.37 - 652156 5.1.3.37 erxpression in Escherichia coli 733323 5.1.3.37 expression in Escherichia coli 733283, 733462, 734053, 734128, 734129, 734293 5.1.3.37 expression of the gene encoding for the isoform AlgE6 R2 module, residues 534-693, in Escherichia coli 733474 5.1.3.37 gene algE4, recombinant expression in Escherichia coli strain ER2566, and expression of chimeric enzyme mutant AlgE64 748180 5.1.3.37 gene algE6, recombinant expression in Escherichia coli strain ER2566, and expression of chimeric enzyme mutant AlgE64, recombinant expression of the R-modules, AlgE6R1, AlgE6R2, and AlgE6R3 748180 5.1.3.37 gene c5epi, DNA and amino acid sequence determination and analysis revealing eight partial amino acid sequences, SjC5-I to -VIII, highest frequency of clone SjC5-VI is found and elucidated with respect to full-length cDNA and putative gene structure, sequence comparisons, RT-PCR enzyme expression analysis in brown algae, functional heterologous expression of His-tagged MC5E clone SjC5-VI in Spodoptera frugiperda Sf9 cells via baculovirus transfection system, and protein secretion 746698 5.1.3.37 gene MEP13, genetic structure, phylogenetic tree, recombinant expression of the codon-optimized His-tagged catalytic domain, MEP13-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL in inclusion bodies. The usage of Escherichia coli strain Rosettagami2(DE3)pLysS produces larger and more significant inclusion bodies than Escherichia coli strain BL21CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP18, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP18-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP2, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP2-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP21, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP21-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP25, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP25-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP26, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP26-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP27, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP27-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP28, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP28-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP29, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP29-C5, in Escherichia coli strain BL 21CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP4, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP4-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP6, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP6-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 gene MEP7, genetic structure, phylogenetic tree, recombinant expression of the His-tagged catalytic domain, MEP7-C5, in Escherichia coli strain BL21 CodonPlus(DE3)RIPL 747915 5.1.3.37 recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha 747457