4.4.1.17 - 94056, 94060 4.4.1.17 cloning in Escherichia coli 651429 4.4.1.17 cloning of the gene CaCYC3 in Escherichia coli 653258 4.4.1.17 coexpression of N-terminally GST-tagged wild-type enzyme with endogenous heme and in complex with its cognate human apocytochrome c and mutants in Escherichia coli 730827 4.4.1.17 coexpression of the wild-type enzyme with periplasmic targeting sequence and addition of the Pseudomonas aeruginosa cytochrome c551/552 pre-sequence, and mutant enzymes with Equus caballus heart cytochrome c or Strep-tagged Saccharomyces cerevisiaecytochrome c in Escherichia coli strain BL21(DE3) 729740 4.4.1.17 expression in Escherichia coli 651850, 652352, 652487 4.4.1.17 expression of the enzyme in Escherichia coli cytosplasm, coexpression with several mutant variants of holocytochrome c552 from Hydrogenobacter thermophilus, the latter are expressed in the periplasm, or several cytochrome c variants from Equus caballus 730322 4.4.1.17 heterologous expression in a multicopy yeast vector 652487 4.4.1.17 Neurospora crassa 94060 4.4.1.17 recombinant coexpression of GST-tagged wild-type and mutant enzymes with cytochrome c in Escherichia coli strain RK103 membranes 729264 4.4.1.17 recombinant coexpression of the GST-tagged enzyme with cytochrome c mutants, containing individual cysteine, histidine, double cysteine, and triple cysteine/histidine substitutions of conserved motif CXXCH, in Escherichia coli strain RK103 membranes, single and double mutants form a complex with the enzyme but not the triple mutant 730058 4.4.1.17 recombinant expression of His-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) cytoplasm, coexpression with Equus caballus cytochrome c 729727 4.4.1.17 Saccharomyces cerevisiae gene CYC3 94056