4.2.1.78 expressed in Escherichia coli BL21(DE3) cells 729604, 730988 4.2.1.78 expression in Escherichia coli and in Saccharomyces cereviseae 749327 4.2.1.78 expression in Escherichia coli BL21 (DE3) 747372 4.2.1.78 expression in Escherichia coli BL21 (DE3). Under certain conditions lyophilized whole Escherichia coli cells containing the overexpressed enzyme turn out to a suitable catalysts 747372 4.2.1.78 expression in Escherichia coli is optimized 682682 4.2.1.78 expression of CjNCS1 in Escherichia coli 680879 4.2.1.78 expression of CjPR10A in Escherichia coli 680879 4.2.1.78 N-terminally truncated enzyme (CjNCS-DELTA29, with a deletion of the first 29 amino acid residues) is expressed in Escherichia coli. The cell-free extract of Escherichia coli expressing CjNCS-DELTA29 exhibits 40fold higher activity than that expressing intact enzyme 747310 4.2.1.78 NCS2-green fluorescent protein fusion introduced by microprojectile bombardment into opium poppy cells initially localizes to the endoplasmic reticulum but subsequently sorts to the vacuole 716518 4.2.1.78 overexpressed in Escherichia coli as a recombinant His-tagged enzyme 678219 4.2.1.78 recombinant expression of C-terminally His-tagged NCS in Eschericha coli strain BL21(DE3) 715141 4.2.1.78 the NCS protein truncated at the first 19 amino acids with an N-terminal MTGS sequence and a His tag at the C-terminus and its SeMet-substituted variant are subcloned into the NdeI and XhoI restriction sites of the vector pET22b. Transformation of chemically competent Escherichia coli strain BL21DE is performed and transformed cells are plated onto LB-ampicillin plates 690258 4.2.1.78 the NCS protein truncated at the first 19 amino acids, with a N-terminal MTGS sequence and a His tag at the C terminus is subcloned into the NdeI and XhoI restriction site of the vector pET22-b. Transformation of chemically competent Escherichia coli strain BL21DE3 693259 4.2.1.78 unlabelled and 15N-labelled delta 29NCS is expressed using the expression system Escherichia coli Rosetta(DE3)/pET29b-delta 29NCS 690826