4.2.1.17 - 4.2.1.17 co-expression of His-tagged alpha- and beta-subunits in Escherichia coli strain Rosetta-2(DE3) 4.2.1.17 expressed in Escherichia coli BL21 4.2.1.17 expressed in Escherichia coli Rosetta 2 (DE3) cells 4.2.1.17 expressed in Escherichis coli BL21(DE3) 4.2.1.17 expression in Escherichia coli 4.2.1.17 expression in Pichia pastoris 4.2.1.17 expression of a His6-tagged truncated ECH2 mutant enzyme 4.2.1.17 expression of recombinant His6-tagged FadB from pET21b-fadBBs in Escherichia coli strain BL21(DE3) 4.2.1.17 gene dspI, quantitative reverse transcriptase PCR expression analysis. Gene dspI is co-transcribed with the upstream genes PA14_54620 and PA14_54630 4.2.1.17 gene echA12 2, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His-tagged enzyme in Escherichia coli in inclusion bodies 4.2.1.17 gene ECHS1, located on chromosome 10, genotyping 4.2.1.17 gene phaJ, overexpression in strain KCTC1639 leads to oversupplementation with (R)-3-hydroxyalkanoate monomers and increased biosynthesis of medium-chain-length polyhydroxyalkanoate 4.2.1.17 recombinant expression of His-tagged AtMFP2 in Escherichia coli strain BL21(DE3) for the wild-type enzyme, and in Escherichia coli strain B834(DE3) for the selenomethionine-labeled variant