3.6.5.4 - 644169, 644176, 657913, 658538, 686396, 687905, 688395, 690025 3.6.5.4 a construct of Thermus aquaticus FtsY in which the first 20 amino acids are deleted is subsequently expressed 688692 3.6.5.4 cpFtsY is cloned and expressed in Escherichia coli 686752 3.6.5.4 expressed in Escherichia coli 698898 3.6.5.4 expressed in Escherichia coli B834(DE3)-Rosetta2 cells 700888 3.6.5.4 expressed in Escherichia coli BL21 (DE3) cells 699552 3.6.5.4 expressed in Escherichia coli BL21 cells 696602 3.6.5.4 expressed in Escherichia coli BL21(DE3) cells 701200 3.6.5.4 expressed in Escherichia coli BL21(DE3)-Rosetta2 cells 700889 3.6.5.4 expressed in Escherichia coli BL21-AI cells 695328 3.6.5.4 expressed in Escherichia coli BL21-CodonPlus(DE3) cells and in COS-1 cells 696594 3.6.5.4 expression in Escherichia coli 644165, 644170, 644172, 644174, 644175, 644178, 644179, 644180, 669412 3.6.5.4 expression of wild-type and mutant cpSRP54 in Escherichia coli strain Rosetta BL21 718911 3.6.5.4 Ffh NG is expressed 684156 3.6.5.4 gene CPSRP54, recombinant overexpression of His6-tagged signal recognition particle protein cpSRP54 in Escherichia coli strain Rosetta, coexpression with signal recognition particle activator protein cpFtsY forming the cpSRP54-cpFtsY complex 757189 3.6.5.4 gene CTHT_0050420, sequence comparisons 758481 3.6.5.4 genes ftsY and ffh, sequence comparisons 757514 3.6.5.4 into the vectors pTD37 and pET22b 687902 3.6.5.4 N and G domains of Ffh, expression in Escherichia coli BL21(DE3)-Rosetta 660000 3.6.5.4 recombinant expression of C-terminally His6-tagged wild-type and mutant mature cpSRP54 (residues 76-564) in Escherichia coli strain BL21 Star 756198 3.6.5.4 recombinant expression of His-tagged chimeric enzyme constructs in Escherichia coli strain BL21 (DE3) 757758 3.6.5.4 recombinant expression of His-tagged chimeric enzyme constructs in Escherichia coli strain BL21(DE3) 757758 3.6.5.4 recombinant large-scale production of all human SRP components and the reconstitution of homogeneous signal recognition particle (SRP) and signal recognition particle receptor (SR) complexes 757857 3.6.5.4 the Escherichia coli strain TOP10 is used for general cloning stategies and the strain SCS110 as an intermediary host in Bacillus cereus transformation experiments, the vectors pRN5101 and pDG148 are used 688862 3.6.5.4 the NG domain of Ffh is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells 684117 3.6.5.4 the NG domain of FtsY is cloned into pUCm-T, and into pET-15b for expression in Escherichia coli BL21 cells 684117 3.6.5.4 the NG domain of Thermus aquaticus Ffh is cloned and subsequently expressed 688692 3.6.5.4 the vector pJGS3 expressing the protein FtsY NGd20, in which the N-terminal residues 2-20 of FtsY are deleted, is constructed by PCR-based site-directed mutagenesis of pTB88 for expression in Escherichia coli Rosetta-2DE3 pLysS cells 689926