3.5.1.53 expressed in Escherichia coli 3.5.1.53 expressed in Escherichia coli to form C-terminal (His)6-tagged protein 3.5.1.53 expression in Escherichia coli strain DE3 3.5.1.53 expression in Escherichia coli, His6-tag 3.5.1.53 overexpression of gene aphA in Escherichia coli