3.4.22.49	-	664553	
3.4.22.49	analysis of correlation between overexpression of separase and aneuploidy in tetracycline-inducible diploid FSK3 mouse mammary epithelial cells	700945	
3.4.22.49	continuous overexpression of cmyc tagged TbSep is lethal to the cells	700280	
3.4.22.49	depletion of endogenous separase with siRNA in NSCLCs shows that separase affects progression through the G2 phase of mitosis. Analysis of the effect of separase depletion on irradiated cells (cell cycle progression, M-phase fraction, late mitotic-linked cell death and clonogenic survival)	697184	
3.4.22.49	enhanced cyclin-dependent kinase 1 activity due to deregulation of CDC27-anaphase-promoting complex leads in turn to hyperphosphorylation of Separase, impeding chromatid separation. Regulation of mitotic progression by transforming growth factor-beta in bone marrow stromal cells is through targeting the APC-separase pathway	700096	
3.4.22.49	expressed in HEK-293T cells	707105, 732187	
3.4.22.49	expressed in HeLa cells	731697, 732505	
3.4.22.49	expression in Escherichia coli strain BL21(DE3)	754886	
3.4.22.49	expression of myc6-separase in 293T cells	638863	
3.4.22.49	expression of wild-type and C2029 mutant enzyme in 293 EcR and HeLa cells	638869	
3.4.22.49	generation of separase gene knock-out mutants	697171	
3.4.22.49	investigation of the expression level of separase across a wide range of human tumors. Overexpression of separase transcripts strongly correlates with high incidence of relapse, metastasis and lower 5-year overall survival rate in breast and prostate cancer patients.	697382	
3.4.22.49	mutations introducing substitutions throughout the Esp1 polypeptide prevent loss of sister chromatid cohesion and cause mitotic failure. Generation of esp1-temperature sensitive mutant for functional analysis of further enzymes	698119	
3.4.22.49	the active (S1126A/T1346A) and the inactive (C1129S) separase mutants are coexpressed in HEK-293T cells with human securin	709220	
3.4.22.49	Zds1 is required for Separase-induced Cdc14 activation. Analysing the role of proteins Zds1 and Zds2 in mitotic exit machinery	699095