3.4.22.10 gene speB, native expression pattern, expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha 670124 3.4.22.10 genes speB and spi, nucleotide and amino acid sequence determination, analysis and comparison, co-expression of immature Spe B and Spi in Streptococcus pyogenes, Spi is similar to the pro-peptide of Spe B, overview, overexpression of His-tagged Spe B propeptide and SpeB mutant C47S in Escherichia coli 670312 3.4.22.10 IdeS GST-fusion protein is expressed in Escherichia coli BL21 700243 3.4.22.10 production of large quantities of soluble streptopain using the autoinduction method for Escherichia coli recombinant protein expression with a C-terminal His6 tag. Soluble streptopain is obtained as mature enzyme, indicating that at some point during autoinduction or cell lysis, the protease must have become active. If incubated long enough after lysis, the protease digestes most proteins in the lysate 755296