3.1.26.12 - 714789, 716266 3.1.26.12 DNA and amino acid sequence determination and analysis, sequence comparisons, expression in Escherichia coli 690026 3.1.26.12 expressed as a fusion with an N-terminal His6 tag in Escherichia coli strain BL21 (DE3) 725381 3.1.26.12 expressed in Escherichia coli 692383 3.1.26.12 expressed in Escherichia coli BL21(DE3) cells 694243, 730942 3.1.26.12 expressed in Escherichia coli DG5alpha and BL21(DE3) cells 730815 3.1.26.12 expressed in Escherichia coli DH5alpha cells 729037 3.1.26.12 expressed in Escherichia coli JM109 cells 729937 3.1.26.12 expressed in Escherichia coli strain BZ99 692391 3.1.26.12 expression in wild-type rne cells and in thermosensitive mutant rne-50 and rne-3071 cells, overexpression of truncated 110 kDa mutant enzyme in rne50 cells 670326 3.1.26.12 expression of His-tagged full-length wild-type RNase E and His-tagged N-terminal ribonucleolytic domain RTD-RNase E, residues 1-498, in strain BL21(DE3) 670423 3.1.26.12 expression of His-tagged N-terminal catalytic domain in strain BL21(DE3) 669202 3.1.26.12 expression of His-tagged truncated enzyme mutants in Escherichia coli strain BL21(DE3), complementation of rne null mutation of Escherichia coli strain KSL2000 687518 3.1.26.12 expression of His-tagged truncated enzyme mutants in strain BL21(DE3), complementation of rne null mutation of strain KSL2000 687518 3.1.26.12 expression of His-tagged wild-type and mutant catalytic domains 670376 3.1.26.12 expression of N-terminal enzyme half, comprising residues 1-499, as C-terminally His6- and Myc-tagged or maltose-binding protein-fused protein in strain BL21(DE3) 670667 3.1.26.12 expression of N-terminally His6-tagged wild-type and mutant full-length enzymes, and of isolated N-terminally His6-tagged S1 domain comprising residues 35-125 669834 3.1.26.12 expression of the His-tagged N-terminal RNaseE catalytic N domain, comprising residues 1-529, in strain BL21(DE3) 667576 3.1.26.12 expression of wild-type and mutant enzyme and N-terminal catalytic domains in Escherichia coli 685062 3.1.26.12 expression of wild-type and mutant enzymes 670301 3.1.26.12 expression of wild-type and mutant enzymes in different modified strains, cell growth curves, overview 668734 3.1.26.12 FLAG-tagged RNase E is expressed in Escherichia coli BL21(DE3) cells 694921 3.1.26.12 fragments of RNase E, either containing the enolase-binding microdomain (residues 833-851), the PNPase-binding site (residues 844-1061) or both (residues 734-1061) 707561 3.1.26.12 gene rne, expression of His-tagged N-terminal half of RNase E in strain BL21(DE3) 669144 3.1.26.12 gene rne, expression of the isolated, His-tagged catalytic domain Rne498, comprising residues 1-498, in strain BL21(DE3) 670424 3.1.26.12 gene rne, expression of wild-type and mutant enzymes in Escherichia coli and in the Saccharomyces cerevisiae two-hybrid system 689760 3.1.26.12 gene rne, overexpression in Escherichia coli, functional complementation of the RNase E-deficient strain CJ1832 of Escherichia coli 669074 3.1.26.12 gene rne, overexpression in strain BL21(DE3) 670712 3.1.26.12 gene rne, overexpression in strain BL21(DE3) as His-tagged enzyme 670721 3.1.26.12 gene rne-1 685655 3.1.26.12 gene rng, overexpression of His10-tagged RNase E/G, Rng, in Escherichia coli strain BL21(DE3) 667401 3.1.26.12 oligohistidine-tagged polypeptides corresponding to the N-terminal half of RNase E with wild-type or mutant sequences 710001 3.1.26.12 overexpressed in Escherichia coli ER2566 706936 3.1.26.12 overexpression of active His- and Myc-tagged truncated enzyme in strain BL21(DE3) 669074 3.1.26.12 overexpression of His10-tagged enzyme in Escherichia coli strain BL21(DE3) 667401 3.1.26.12 overexpression of plasmid-encoded rne gene increases cell doubling time and can lead to plasmid loss or aquisition of mutations that reduce RNase E activity 670298 3.1.26.12 overexpression of wild-type and truncation mutant enzymes in strain Bl21(DE3), expression in the yeast two-hybrid system, overview 668733 3.1.26.12 recombinant expression of FLAG-tagged truncation mutants 716262 3.1.26.12 recombinant overexpression of the His-tagged enzyme in Escherichia coli 716388 3.1.26.12 rne gene amplified and subcloned into pCR-BluntII-TOPO vector to generate pTOPOS14Rne. Expression plasmid pBADS14Rne constructed by excising the rne gene as a 3.3 kb gene Nco I-Pme I fragment from pTOPOS14Rne and inserting the fragment into the medium copy number plasmid pBADlacZ, putting RNase E expression under the control of the arabinose inducible promoter PBAD, and transformed into Escherichia coli AC23 (rne-1). RNase E putative enolase-binding microdomain (residues 885-909) and the putative PNPase-binding microdomain from S14 RNase E (residues 1015-1094) cloned 707561