3.1.2.22	expressed in COS-1 cells	23911	
3.1.2.22	expression in endothelial cells	23919	
3.1.2.22	baculovirally expressed	23920	
3.1.2.22	DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene	651030	
3.1.2.22	expression of wild-type and enzymes with naturally occurring mutations in COS cells as His-tagged enzyme, and in Sf9 cells, the latter via baculovirus infection	651450	
3.1.2.22	expression as glutathione S-transferase fusion protein in BHK and CHO cells, localization of the recombinant enzyme out side the lysosomes and endosomes	651451	
3.1.2.22	expression of the enzyme in Caco-2 cells, orientation to the basolateral side, also after endocytosis of the recombinant enzyme into Caco-2 cells	651451	
3.1.2.22	-	23913, 651455, 729768	
3.1.2.22	gene ppt, DNA sequence determnation and analysis, identification of 8 natural mutations of different origins leading to enzyme deficiency	651456	
3.1.2.22	overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue	652459	
3.1.2.22	expression of enzyme carrying naturally occurring mutations in COS-1 cells	653226	
3.1.2.22	expression of wild-type and mutant in COS-1 cells	653651	
3.1.2.22	PPT1-KO mice are generated by gene targeting in ES cells	693353	
3.1.2.22	GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell	707548	
3.1.2.22	PPT1-deficient fibroblasts stably transfected with cDNA encoding PPT1	707557	
3.1.2.22	fragment corresponding to the entire coding region of PPT1 amplified from pCMV5-hPPT1, and cloned into XhoI sites in the polylinker region of expression vector pMSXND1, overexpressed in CHO cells. PPT1 injected intravenously into PPT1-deficient mice	709975	
3.1.2.22	construction of a UAS:GFP-Ppt1 transgenic fly line. The pUAST:GFP-Ppt1 vector overexpressed using Act5C-Gal4	710070	
3.1.2.22	overexpressed in Drosophila melanogaster	710070	
3.1.2.22	expressed in Mus musculus	751433	
3.1.2.22	expressed in COS-7 and HEK-293 cells	751937