3.1.2.22	-	23913, 651455, 729768	
3.1.2.22	baculovirally expressed	23920	
3.1.2.22	construction of a UAS:GFP-Ppt1 transgenic fly line. The pUAST:GFP-Ppt1 vector overexpressed using Act5C-Gal4	710070	
3.1.2.22	DNA sequence determination and analysis, enzyme is encoded in frame with a second gene coding for an indispensable dolichol pyrophosphatase, gene pdf1, strains bearing a deletion of the entire pdf1 open reading frame are nonviable, due to Dolpp1p deficiency, and are rescued by pdf1 expression plasmid, mutations only in the palmitoyl protein thioesterase 1 domain results in cells abnormally sensitive to sodium vanadate and elevated extracellular pH, the pdf1-deficient phenotype is complemented by the human PPT1 gene	651030	
3.1.2.22	expressed in COS-1 cells	23911	
3.1.2.22	expressed in COS-7 and HEK-293 cells	751937	
3.1.2.22	expressed in Mus musculus	751433	
3.1.2.22	expression as glutathione S-transferase fusion protein in BHK and CHO cells, localization of the recombinant enzyme out side the lysosomes and endosomes	651451	
3.1.2.22	expression in endothelial cells	23919	
3.1.2.22	expression of enzyme carrying naturally occurring mutations in COS-1 cells	653226	
3.1.2.22	expression of the enzyme in Caco-2 cells, orientation to the basolateral side, also after endocytosis of the recombinant enzyme into Caco-2 cells	651451	
3.1.2.22	expression of wild-type and enzymes with naturally occurring mutations in COS cells as His-tagged enzyme, and in Sf9 cells, the latter via baculovirus infection	651450	
3.1.2.22	expression of wild-type and mutant in COS-1 cells	653651	
3.1.2.22	fragment corresponding to the entire coding region of PPT1 amplified from pCMV5-hPPT1, and cloned into XhoI sites in the polylinker region of expression vector pMSXND1, overexpressed in CHO cells. PPT1 injected intravenously into PPT1-deficient mice	709975	
3.1.2.22	gene ppt, DNA sequence determnation and analysis, identification of 8 natural mutations of different origins leading to enzyme deficiency	651456	
3.1.2.22	GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell	707548	
3.1.2.22	overexpressed in Drosophila melanogaster	710070	
3.1.2.22	overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue	652459	
3.1.2.22	PPT1-deficient fibroblasts stably transfected with cDNA encoding PPT1	707557	
3.1.2.22	PPT1-KO mice are generated by gene targeting in ES cells	693353