2.7.1.173 gene NMRK1 2.7.1.173 gene NMRK2 2.7.1.173 gene NRK1, quantitative real-time PCR enzyme expression analysis 2.7.1.173 His-tagged wild-type Nrk1 expression in Escherichia coli, expression of wild-type and mutant Nrk1 from the GAL1 promoter on a LEU2 plasmid in Saccharomyces cerevisiae strain BY278 2.7.1.173 His-tagged wild-type Nrk2 expression in Escherichia coli, expression of wild-type and mutant Nrk2 from the GAL1 promoter on a LEU2 plasmid in Saccharomyces cerevisiae strain BY278 2.7.1.173 overexpression of His-tagged NRK1 in Escherichia coli strain BL21(DE3)