2.3.1.39 a hexa-histidine-tagged enzyme overexpressed in Escherichia coli 486892 2.3.1.39 cloning and nucleotide sequence analysis of the enzyme gene, overexpression of fabD89 gene in Escherichia coli 486900 2.3.1.39 cloning and sequencing of the enzyme gene using equivalent gene of Escherichia coli HB101 as a probe. The overexpression of the enzyme gene induces monomer supply for polyhydroxybutyrate production in Escherichia coli HB101 486894 2.3.1.39 cloning and sequencing of the enzyme gene, expressed in Escherichia coli BL21 486895 2.3.1.39 cloning, nucleotide sequence and expression of the fabD-gene in an appropriate Escherichia coli expression vector 486884, 486885 2.3.1.39 DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) 661063 2.3.1.39 DNA and amino acid sequence determination and analysis, phylogenetic tree, expression as GFP-fusion protein containing the mitochondrial targeting sequence in mitochondria of HeLa cells, expression of truncated enzyme forms lacking the first 21 or 59 amino acid residues, respectively, in Escherichia coli strain BL21(DE3) as soluble enzymes, or in Spodoptera frugiperda Sf9 cells via baculovirus infection system 659451 2.3.1.39 effects of overexpression on the fatty acid composition of the membrane phospholipids of Escherichia coli 486885 2.3.1.39 expressed as a His-tagged fusion protein 688366 2.3.1.39 expressed as a His-tagged fusion protein in Escherichia coli 689905 2.3.1.39 expressed in Escherichia coli 719494, 720409, 720468 2.3.1.39 expressed in Escherichia coli as a hexahistidine-tagged fusion protein 486891 2.3.1.39 expressed in Escherichia coli as a His-tagged fusion protein 684213 2.3.1.39 expressed in Escherichia coli BL21(DE3) cells 755972 2.3.1.39 expressed in HEK-239T, COS7 and Hela cells 756900 2.3.1.39 expressed in Nicotiana benthamiana leaf protoplasts 757986 2.3.1.39 expression in Escherichia coli 701486, 701546 2.3.1.39 expression in Escherichia coli of cDNAs of various lengths encoding the second domain of EC 2.3.1.85. Expression of a shortened transacylase, consisting of EC 2.3.1.85 residues 488-809 in Spodoptera frugiperda 486896 2.3.1.39 expression in Escherichia coli strain BL21(DE3) 661925 2.3.1.39 expression in Saccharomyces cerevisiae mutant lacking the corresponding mitochondrial malonyl-CoA transerase Mctp1 703315 2.3.1.39 expression of five mutants in Escherichia coli 486893 2.3.1.39 expression of mutant proteins R606A and R606K in Escherichia coli 486873 2.3.1.39 expression of the enzyme gene in Escherichia coli 486898 2.3.1.39 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 661061 2.3.1.39 fabd2 is inserted into a bacterial expression vector pET28a resulting in a 6' histidine-tag fabd2 fusion gene construction 676953 2.3.1.39 from K-12 strain MC4100, overexpression in strain M15 660689 2.3.1.39 gene fabD or slr2023, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain DH5alpha, recombinant expression of the His6-tagged enzyme in Escherichia coli strain BL21 (DE3) 735381 2.3.1.39 gene fabD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant overexpression of GST-tagged enzyme in Saccharomyces cereviseae strain INVScl significantly increasing the MCAT activity, without negative effects on the growth rate of the host strain 737303 2.3.1.39 gene fabD, expression of His-tagged enzyme in Escherichia coli strain BL21 663400 2.3.1.39 gene fabD, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 735606 2.3.1.39 gene NgMCAT, DNA and amino acid sequence determination and analysis, phylogenetic analysis, realtime quantitative PCR expression analysis 736175 2.3.1.39 subcloned into NdeI and XhoI sites of the pET28a expression vector, and transformed into Escherichia coli BL21 (DE3) 755972