2.3.1.167	-	756740	
2.3.1.167	expression in Escherichia coli	486453, 755829, 757640, 757765	
2.3.1.167	gene dbat, functional expression in Escherichia coli strain JM109, co-expression with acetyl-CoA synthase, gene acs, EC 6.2.1.1, or with acetoacetate:acetyl-CoA CoA-transferase, genes atoAD of the ato operon, EC 2.8.3.8, expressed from and regulated by genes encoded on the bacterial chromosome, overview	672726	
2.3.1.167	into the TA cloning vector pMD19-T for sequencing	685463, 686959	
2.3.1.167	into the vector pCWori+ for expression in Escherichia coli JM109 and BL21DE3 cells	672726	
2.3.1.167	overexpression in Escherichia coli. Three kinds of fusion systems with isopropyl-beta-D-1-thiogalactopyranoside inducible promoters are used. The fusion partners are glutathione S-transferase tag, hexahistidine fusion tag, and a biotinylated peptide tag	660432	
2.3.1.167	the amplified PCR fragment is subcloned into the pGEM-T Easy vector and subsequently into the pET32a+ vector for expression in Escherichia coli BL21DE3 cells	688912