2.1.1.74	expressed in Escherichia coli as a His-tagged fusion protein	
2.1.1.74	expression in Escherichia coli	
2.1.1.74	overexpression in Escherichia coli strain BL21(DE3)	
2.1.1.74	the encoded recombinant protein contains tightly bound flavin and is active in Escherichia coli mutant lacking m5U-54 in tRNAs and in vitro using T7 tRNA transcript as substrate	
2.1.1.74	untagged N-terminus and C-terminus (His)6-tagged TrmFO from Bacillus subtilis is expressed in Escherichia coli. The tag does not significantly alter the expression level, flavin content, activity and secondary structure of the protein