1.3.8.5 enzyme form ECRI 1.3.8.5 expression in Escherichia coli strain XL1Blue 1.3.8.5 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 1.3.8.5 full-length cDNA clones of both reductase isoforms isolated, cloned, and sequenced 1.3.8.5 full-length cDNA coding for the major isoform of the enzyme, ECRI, cloned and sequenced and compared with that of the minor isoform of the enzyme, ECRII 1.3.8.5 the enzyme forms ECRI and ECRII appear to be encoded by separate genes