1.2.4.4 - 349040, 349083, 349084, 349085, 674648, 675563 1.2.4.4 beta-subunit 349049, 349050, 349089 1.2.4.4 expressed in Escherichia coli BL-21 cells 687543 1.2.4.4 expressed in Escherichia coli BL21(DE3) cells and BL21(DE3)pLysS cells 686676 1.2.4.4 expressed in Escherichia coli strain DH5alpha 681761 1.2.4.4 expression in Escherichia coli 349086, 762852 1.2.4.4 expression in Pseudomonas putida and Escherichia coli 349048 1.2.4.4 expression of alpha- and beta-subunits of component E1 in Escherichia coli 656534 1.2.4.4 expression of C-terminally His-tagged lipoic acid-bearing domain LBD, amino acid residues 1-84 of E2b 656171 1.2.4.4 expression of C-terminally His-tagged lipoic acid-bearing domain of enzyme complex component E2 with an extra methionine at the N-terminus and a glutamic acid and leucine at the C-terminus in Escherichia coli strain BL21(DE3) 655560 1.2.4.4 expression of E1 component in Escherichia coli as a homotetramer, in Escherichia coli only one translational start site for E1beta is recognized and only the 39000 Da product is produced, in addition the N-terminal amino acid of the 39000 Da E1beta subunit is Met instead of Leu 349082 1.2.4.4 expression of N-and C-terminally His6-tagged component E1b and mutants, and of C-terminally His-tagged lipoic acid-bearing domain, amino acid residues 1-84 of component E2b, in vitro lipoylation of the latter 656230 1.2.4.4 expression of the lipoic acid-bearing domain of complex component E2 in Escherichia coli strain BL21(DE3) as His-tagged protein 656045 1.2.4.4 overexpression of bacterial branched-chain alpha-keto acid dehydrogenase multienzyme complex BCKDC, encoded by 4 genes, in Arabidopsis thaliana targeted to chloroplasts, about 7fold increased enzyme activity in the transgenic plants 657104 1.2.4.4 recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with superoxide dismutase of Thermus thermophilus in Escherichia coli, interaction analysis of 2-oxoisovalerate dehydrogenase alpha subunit with superoxid dismutase of Thermus thermophilus by yeast two hybrid system, recombinant expression in Saccharomyces cerevisiae 742612 1.2.4.4 region containing the genes for the entire enzyme complex 349047, 349048