1.17.1.3 - 636457 1.17.1.3 determination of DNA and amino acid sequences, expression in Escherichia coli strain XL-1 Blue, Nicotiana tabacum, and Trifolium repens, plant transformations via Agrobacterium tumefaciens infection system 636457 1.17.1.3 ectopic expression of CsLAR leads to the formation of (+)-catechin, but also of (-)-epicatechin in anthocyanin producing tissues of tobacco plants 745825 1.17.1.3 expression in Escherichia coli strain BL21(DE3) 654366 1.17.1.3 expression in Escherichia coli strain DH5alpha, expression as MBT-fusion protein followed by cleavage of the protein tag by Factor Xa protease 654366 1.17.1.3 expression of His-tagged LAR1 in Escherichia coli 715907 1.17.1.3 gene DkLAR, DNA and amino acid sequence determination and analysis, genotyping and phylogenetic analysis. Expression of the DkLAR gene in Chinese pollination-constant non-astringent, PCNA, genotype is coincident with the tannin cell development, but is not in Japanese PCNA and Chinese pollination-variant astringent PCA genotypes 714393 1.17.1.3 gene DkLAR, DNA and amino acid sequence determination and analysis, genotyping. Expression of the DkLAR gene in Chinese pollination-constant non-astringent, PCNA, genotype is coincident with the tannin cell development, but is not in Japanese PCNA and Chinese pollination-variant astringent PCA genotypes 714393 1.17.1.3 gene LAR, co-overexpression with anthocyanidin reductase (ANR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis 743899 1.17.1.3 gene lar, DNA and amino acid sequence determination and analysis, sequence comaprisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis 746499 1.17.1.3 gene lar, overexpression of the enzyme in Medicago truncatula hairy roots, conversion of 4beta-(S-cysteinyl)-epicatechin to epicatechin by recombinant LAR 745869 1.17.1.3 gene LAR, quantitative real-time PCR enzyme expression analysis 744993 1.17.1.3 gene LAR1, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis 745634 1.17.1.3 gene LAR1, quantitative real-time PCR enzyme expression analysis, recombinant expression in Nicotiana tabacum cv. Petite Havana SR1 using the transfection method with Agrobacterium tumefaciens strain GV3101 and under control of the CaMV 35S promoter. The proanthocyanidin contents in either white- or pale pink-colored transgenic flowers are significantly lower than that of wild-type flowers. In contrast, both pale-pink and white flowers of the transgenic lines accumulate slightly higher levels of epicatechin than wild-type flowers, but the changes do not reach statistical significance. No significant change in catechin content is observed between wild-type flowers and either white- or pale pink-colored transgenic flowers, phenotypes, overview 744993 1.17.1.3 gene LAR2, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis 745634 1.17.1.3 gene LAR3, cloned from leaves, DNA and amino acid sequence determination and analysis, genotyping, the three LAR loci, FeLAR1, FeLAR2, and FeLAR3, are not genetically linked, phylogenetic analysis 745634 1.17.1.3 gene LAR3, DNA and amino acid sequence determination, genotyping, genetic organization and allelic structure of the gene, identification of nonsynonymous substitutions, and to analysis of motifs in the promoter, recombinant expression in Nicotiana benthamiana, and quantitative RT-PCR analyis of allele-specific gene expression of the PaLAR3 alleles 746116 1.17.1.3 gene LARa, complementary DNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional expression of His6-tagged enzyme in Escherichia coli strain M15. Recombinant ectopic expression of CsLAR leads to the accumulation of low levels of proanthocyanidin precursors and their conjugates in Medicago truncatula hairy roots and anthocyanin-overproducing Nicotiana tabacum, but levels of oligomeric proanthocyanidins are very low. The expression of CsLAR in tobacco overproducing anthocyanin leads to the accumulation of higher levels of epicatechin and its glucoside than of catechin, phenotype and flavonoid compounds contents, overview 746103 1.17.1.3 gene LARa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco 746163 1.17.1.3 gene LARb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco 746163 1.17.1.3 gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco 746163 1.17.1.3 gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco 746163 1.17.1.3 genes Vv lar1 and Vv lar2, quantitative real-time RT-PCR enzyme expression analysis 745035 1.17.1.3 genes Vvlar1 and Vvlar2, quantitative real-time RT-PCR enzyme expression analysis 745035 1.17.1.3 recombinantly expressed in Escherichia coli 727256