1.14.19.34	gene DsFAD2-1, DNA and amino acid sequence determination and analysis, recombinant expression of DsFAD2-1 in Saccharomyces cerevisiae strain YPH499 and Glycine max somatic embryos resulting in the accumulation of the trans-DELTA12 isomer of linoleic acid (18:2
9cis,12trans) rather than the more typical cis-DELTA12 isomer. When co-expressed with DsFAD2-1 in soybean embryos or yeast, DsFAD2-2 converts 18:2
9cis,12trans into dimorphecolic acid	
1.14.19.34	gene DsFAD2-2, DNA and amino acid sequence determination and analysis, recombinant expression of DsFAD2-2 in Saccharomyces cerevisiae strain YPH499 and Glycine max somatic embryos. When co-expressed with DsFAD2-1 in soybean embryos or yeast, DsFAD2-2 converts 18:2
9cis,12trans into dimorphecolic acid. When DsFAD2-2 is expressed alone in soybean embryos or together with a typical cis-DELTA12-oleic acid desaturase in yeast, trace amounts of the cis-DELTA12 isomer of dimorphecolic acid (9-OH-18:2DELTA9cis,12cis) are formed from DsFAD2-2 activity with cis-DELTA12-linoleic acid	
1.14.19.34	gene FAD2, combined transgenic expression of Punica granatum conjugase (FADX, EC 1.14.19.33) under the control of a seed-specific napin promoter and Arabidopsis thaliana FAD2 desaturase in high linoleic acid Arabidopsis thaliana fad3/fae1 mutant, via Agrobacterium tumefaciens cell strain GV3101 transformation, leads to increased accumulation of punicic acid, quantitative RT-PCR enzyme expression analysis	
1.14.19.34	gene FAD2, DNA and amino acid sequence determination and analysis, functional expression of the enzyme in Saccharomyces cerevisiae strain MMYO11,
transient expression of myc-tagged enzyme in Nicotiana tabacum cv. Bright-Yellow 2 suspension-cultured BY-2 cells