1.14.18.3 - 712040 1.14.18.3 DNA and amino acid sequence determination and anaylsis, the gene is organized in the structural gene cluster pmoCAB, stable functional expression of the membrane-bound enzyme in Rhodococcus erythropolis strain LSSE8-1 by using the dsz promoter and ethane as the sole carbon source, method optimization, overview 673739 1.14.18.3 expressed in Escherichia coli 438942 1.14.18.3 expressed in Escherichia coli BL21 cells 728079 1.14.18.3 expressed in Escherichia coli BL21(DE3) or Rosetta (DE3) pLysS cells 716109 1.14.18.3 gene pmoA, design of a highly degenerate primer targeting copper-containing membrane-bound monooxygenase genes for community analysis of methane- and ammonia-oxidizing bacteria, two-step PCR strategy employing a tagged highly degenerate primer (THDP), designated THDP-PCR, method development and optimization, overview. DNA and amino acid sequence determination and analysis, phylogenetic analysis 744923 1.14.18.3 genetic analysis of pMMO genes 438952 1.14.18.3 pmoA cloned in pGEM-T Easy vector and transformed into Escherichia coli JM109 high-efficiency competent cells 705544 1.14.18.3 recombinant expression of wild-type holoenzyme and truncated recombinant periplasmic domains of pMMO (spmoB) 744325 1.14.18.3 two aqueous-exposed subdomains toward the N- and C-termini of the large subunit are expressed in Escherichia coli BL21 (DE3) cells 685113