3.1.2.22	expression of the enzyme in Caco-2 cells, orientation to the basolateral side, also after endocytosis of the recombinant enzyme into Caco-2 cells	651451	
3.1.2.22	expression of wild-type and enzymes with naturally occurring mutations in COS cells as His-tagged enzyme, and in Sf9 cells, the latter via baculovirus infection	651450	
3.1.2.22	expression of wild-type and mutant in COS-1 cells	653651	
3.1.2.22	fragment corresponding to the entire coding region of PPT1 amplified from pCMV5-hPPT1, and cloned into XhoI sites in the polylinker region of expression vector pMSXND1, overexpressed in CHO cells. PPT1 injected intravenously into PPT1-deficient mice	709975	
3.1.2.22	gene ppt, DNA sequence determnation and analysis, identification of 8 natural mutations of different origins leading to enzyme deficiency	651456	
3.1.2.22	GST-fusion protein expressed in Escherichia coli BL21, expressed in HEK-293 cell	707548	
3.1.2.22	overexpressed in Drosophila melanogaster	710070	
3.1.2.22	overexpression of PPT2 in an insect-baculovirus expression system, secretion of the recombinant enzyme to the culture medium, expression of mutant PPT2 in COS cells, subcloning in Escherichia coli XL1-blue	652459	
3.1.2.22	PPT1-deficient fibroblasts stably transfected with cDNA encoding PPT1	707557	
3.1.2.22	PPT1-KO mice are generated by gene targeting in ES cells	693353