2.1.1.217	additional information	the methyltransferase TrmK (BsTrmK) is responsible for the formation of m1A22 in tRNA. BsTrmK displays a broad substrate specificity, and methylates seven out of eight tRNA isoacceptor families of Bacillus subtilis bearing an A22. In addition to a non-Watson-Crick base-pair between the target A22 and a purine at position 13, the formation of m1A22 by BsTrmK requires a full-length tRNA with intact tRNA elbow and anticodon stem. Measurements of the MTase activity using 32P-radiolabelled Bacillus subtilis tRNAs. Interaction between BsTrmK and the cofactor product S-adenosyl-L-homocysteine (SAH) is enthalpy-driven with a single binding site and a dissociation constant (KD) of 0.0017 mM, residues R9, L10, G77, D78, A94 and G95 are involved in SAH binding	Bacillus subtilis	?	-	-	89	
2.1.1.217	additional information	the methyltransferase TrmK (BsTrmK) is responsible for the formation of m1A22 in tRNA. BsTrmK displays a broad substrate specificity, and methylates seven out of eight tRNA isoacceptor families of Bacillus subtilis bearing an A22. In addition to a non-Watson-Crick base-pair between the target A22 and a purine at position 13, the formation of m1A22 by BsTrmK requires a full-length tRNA with intact tRNA elbow and anticodon stem. Measurements of the MTase activity using 32P-radiolabelled Bacillus subtilis tRNAs. Interaction between BsTrmK and the cofactor product S-adenosyl-L-homocysteine (SAH) is enthalpy-driven with a single binding site and a dissociation constant (KD) of 0.0017 mM, residues R9, L10, G77, D78, A94 and G95 are involved in SAH binding	Bacillus subtilis 168	?	-	-	89	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in Bacillus subtilis tRNA(Ser)	crude Escherichia coli tRNA is used, since it does not contain m1A22	Bacillus subtilis	S-adenosyl-L-homocysteine + N1-methyladenine22 in Bacillus subtilis tRNA(Ser)	-	?	415241	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	-	Streptococcus pneumoniae	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	-	Bacillus subtilis	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	-	Vibrio cholerae	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	-	Listeria monocytogenes serotype 4b	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	-	Staphylococcus aureus	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	a methyl group at position N1 prevents Watson-Crick-type base pairing by adenosine and is therefore important for regulation of structure and stability of tRNA molecules	Bacillus subtilis	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576	
2.1.1.217	S-adenosyl-L-methionine + adenine22 in tRNA	crude Escherichia coli tRNA is used, since it does not contain m1A22	Bacillus subtilis	S-adenosyl-L-homocysteine + N1-methyladenine22 in tRNA	-	?	413576