6.5.1.B3	a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end	-	Pyrococcus furiosus	a mature tRNA molecule containing a 2'-5'-phosphodiester bond	-	?	421829	
6.5.1.B3	a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end	synthetic tRNA halves bearing 2',3'-cyclic phosphate and 5'-OH termini are substrates for the ligation reaction in vitro. The activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity. The enzyme cleaves a 2',3'-cyclic phosphate group to produce a 2'-phosphate group at the 3' terminus of an RNA oligomer	Pyrococcus furiosus	a mature tRNA molecule containing a 2'-5'-phosphodiester bond	the ligation product is unstable during in vitro reaction	?	421829