6.3.3.6 ATP + (2R,5R)-5-carboxymethylproline low activity, kcat/Km is 2% compared to the value for (2S,5S)-5-carboxymethylproline Pectobacterium carotovorum ? - ? 422777 6.3.3.6 ATP + (2S,5R)-5-carboxymethylproline low activity, kcat/Km is 2% compared to the value for (2S,5S)-5-carboxymethylproline Pectobacterium carotovorum ? - ? 422778 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline - Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline - Streptomyces cattleya AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline the enzyme catalyzes the formation of the beta-lactam ring in (5R)-carbapenem-3-carboxylic acid biosynthesis Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline the enzyme is involved in the biosynthesis of the carbapenem beta-lactam antibiotic (5R)-carbapen-2-em-3-carboxylate Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline a mechanism is proposed where the rate-limiting step is beta-lactam ring formation coupled to a protein conformational change. The role of K443 throughout the reaction is undercored Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline catalytic Tyr-Glu dyad is demonstrated by site-directed mutagenesis and kinetic experiments that compare the wild-type enzymes to their respective mutant proteins using pH–rate profiles, 32P-incorporation experiments, solvent isotope effects, proton inventory, and viscosity variation Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,5S)-5-carboxymethylproline the kinetic mechanism is bi-ter where ATP is the first substrate to bind followed by (2S,5S)-5-carboxymethyl proline and diphosphate is the last product released. Low activity with (2S,5R)-5-carboxymethylproline or (2R,5R)-5-carboxymethylproline Pectobacterium carotovorum AMP + diphosphate + (3S,5S)-carbapenam 3-carboxylate - ? 421845 6.3.3.6 ATP + (2S,6R)-2,6-dimethyl-t-carboxymethylproline - Pectobacterium carotovorum AMP + diphosphate + (3S,5S,6R)-3,6-dimethylcabapenam-3-carboxylate - ? 443079