3.4.24.82 5-carboxyfluorescein-Ala-Glu-Leu-Gln-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N',N'-tetramethyl-6-carboxyrhodamine + H2O - Homo sapiens 5-carboxyfluorescein-Ala-Glu + Leu-Gln-Gly-Arg-Pro-Ile-Ser-Ile-Ala-Lys-N,N,N',N'-tetramethyl-6-carboxyrhodamine - ? 449364 3.4.24.82 Abz-TEGEARGSVI-Dap(2,4-dinitrophenyl)-KK + H2O - Homo sapiens ? - ? 391107 3.4.24.82 aggrecan + H2O - Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 100 microg/ml Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 2-8 microg, assay at pH 7.2, 37°C, reaction stopped by addition of 21 mM EDTA Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 2mg/ml, assay at 37°C, 16 h Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O 40 microg Mus musculus ? - ? 15923 3.4.24.82 aggrecan + H2O ADAMTS-4 and ADAMTS-5 are the proteases responsible for the endogenous aggrecanase activity observed in IL-1beta stimulated bovine cartilage Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O cleavage at the Glu373-Ala374 site of aggrecan is measured Homo sapiens ? - ? 15923 3.4.24.82 aggrecan + H2O non-conservative mutation at P2' position of the bovine aggrecan (R375L) does not result in an observable difference in ADAMTS-4 susceptibility. Mutations in recombinant bovine aggrecan at Ser377 to Thr or Asn do not result in any significant difference in ADAMTS-4 cleavage. The S377Q mutation results in a significant reduction of cleavage by ADAMTS-4. The S377Q mutant is susceptible to cleavage by the C-terminally truncated ADAMTS-4. The S377A mutant is cleaved similarly to wild-type aggrecan Homo sapiens ? - ? 15923