3.1.21.3 3'-biotinylated 51 bp dsDNA + H2O - Micrococcus luteus ? - ? 364429 3.1.21.3 5'-biotinylated 51 bp ssDNA + H2O - Micrococcus luteus ? - ? 364430 3.1.21.3 DNA + H2O - Escherichia coli K-12 ? - ? 141228 3.1.21.3 DNA + H2O - synthetic construct ? - ? 141228 3.1.21.3 DNA + H2O - Geobacillus stearothermophilus ? - ? 141228 3.1.21.3 DNA + H2O - Vibrio vulnificus YJ016 ? - ? 141228 3.1.21.3 DNA + H2O EcoR124I couples ATP hydrolysis to bidirectional DNA translocation Escherichia coli ? - ? 141228 3.1.21.3 DNA + H2O the enzyme must overcome a similar slow step before translocation reaches a steady state Escherichia coli ? - ? 141228 3.1.21.3 DNA + H2O R.HpyAXII effectively restricts chromosomal DNA during natural transformation Helicobacter pylori ? - ? 141228 3.1.21.3 DNA + H2O HsdR subunit can produce only a single cleavage of the phosphodiester backbone of the DNA but can cooperate with another HsdR subunit to produce full DNA cleavage, producing DNA with overhanging ends of single-stranded DNA. On a single-site plasmid, cleavage requires the association of HsdR, from solution, to the cleavage complex in order to produce double-strand cleavage Escherichia coli ? - ? 141228