2.4.99.21	an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine	-	Haloferax volcanii	an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	-	?	433419	
2.4.99.21	an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine	-	Archaeoglobus fulgidus	an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	the oligosaccharide chain that Archaeoglobus fulgidus AglB-L transfers to the Asn residues in proteins has the structure alpha-D-Glcp-(1-6)-alpha-[(1-3)-alpha-D-GlcP]-D-Manp-(1-3)-alpha-D-Galp-(1-2)-alpha-D-Manp-(1-3)-[(1-4)-beta-D-Glcp]-beta-D-Galp-(1-4)-beta-D-Glcp-Asn. The monosaccharide residue that is directly linked to the Asn residue is hexose	?	433419	
2.4.99.21	an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine	the Pyrococcus furiosus and Archaeoglobus fulgidus AglBs preferentially glycosylate sequons with Val and Glu at the X position, respectively	Pyrococcus furiosus	an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	-	?	433419	
2.4.99.21	an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine	the Pyrococcus furiosus and Archaeoglobus fulgidus AglBs preferentially glycosylate sequons with Val and Glu at the X position, respectively	Archaeoglobus fulgidus	an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	-	?	433419	
2.4.99.21	dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronosyl)-N-acetyl-alpha-D-glucosaminyl phosphate + Ac-YKYQESSYK-(4-nitro)F-NH2	the peptide is based on the sequence of the natively glycosylated flagellum protein FlaB2. The enzyme shows no activity with the peptide Ac-YKYQESSYK-(4-nitro)F-NH2, lacking the key asparagine residue. Although the full length N-glycan generated by Methanococcus voltae (L-threonyl 2-(acetylamino)-2-deoxy-beta-D-mannuronamido-(1->4)-2,3-bis(acetylamino)-2,3-bis(acetylamino)-2,3-dideoxy-beta-D-glucuronosyl-(1->3)-2-(acetylamino)-2-deoxy-beta-D-glucosyl-[protein]-L-asparagine) is a trisaccharide the disaccharide-linked substrate is sufficient for oligosaccharyl transferase activity	Methanococcus voltae	dolichol + ?	-	?	431825	
2.4.99.21	dolichyl 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronosyl)-N-acetyl-alpha-D-glucosaminyl phosphate + [flagellum protein FlaB2]-L-asparagine	the enzyme is incubated with the acceptor peptide Ac-YKYQESSYK-(4-nitro)F-NH2, which is based the natively glycosylated flagellum protein FlaB2 sequence. The activity is dependent on the canonical AsnXaaSer/Thr sequon. Although the full length N-glycan generated by Methanococcus voltae (L-threonyl 2-(acetylamino)-2-deoxy-beta-D-mannuronamido-(1->4)-2,3-bis(acetylamino)-2,3-bis(acetylamino)-2,3-dideoxy-beta-D-glucuronosyl-(1->3)-2-(acetylamino)-2-deoxy-beta-D-glucosyl-[protein]-L-asparagine) is a trisaccharide the disaccharide-linked substrate is sufficient for oligosaccharyl transferase activity	Methanococcus voltae	dolichol + 3-O-(2,3-diacetamido-2,3-dideoxy-beta-D-glucuronosyl)-N-acetyl-alpha-D-glucosaminyl-[flagellum protein FlaB2]-L-asparagine	-	?	431826	
2.4.99.21	dolichyl phosphooligosaccharide + [flagellum protein FlaB2]-L-asparagine101	the enzyme participates in the N-linked glycosylation in Methanococcus voltae. It catalyzes transfer of oligosaccharides from the dolichyl phosphate donor to asparagine in the acceptor proteins. The activity is dependent on the canonical AsnXaaSer/Thr sequon. Although the full length N-glycan generated by Methanococcus voltae (L-threonyl 2-(acetylamino)-2-deoxy-beta-D-mannuronamido-(1->4)-2,3-bis(acetylamino)-2,3-bis(acetylamino)-2,3-dideoxy-beta-D-glucuronosyl-(1->3)-2-(acetylamino)-2-deoxy-beta-D-glucosyl-[protein]-L-asparagine) is a trisaccharide, the disaccharide-linked substrate is sufficient for oligosaccharyl transferase activity	Methanococcus voltae	dolichol + flagellum protein FlaB2 with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	-	?	430966	
2.4.99.21	dolichyl phosphosulfoheptasaccharide + [protein]-L-asparagine	-	Archaeoglobus fulgidus	dolichyl phosphate + a glycoprotein with the sulfoheptasaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	-	?	436465	
2.4.99.21	lipid-linked oligosaccharide + [peptide]-L-asparagine	-	Archaeoglobus fulgidus	lipid + a glycopeptide with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine	the glycan structure is estimated to be a branched heptasaccharide	?	433910	
2.4.99.21	additional information	the enzyme (AglB) has the ability to attach incomplete glycans as seen by the disaccharides present on flagellins and S-layer proteins in Methanococcus voltae aglA mutant cells. AglA is the glycosyl transferase involved in the attachment of the terminal sugar to the glycan	Methanococcus voltae	?	-	?	89