2.1.1.264 additional information the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY Escherichia coli ? - ? 89 2.1.1.264 additional information the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173 Escherichia coli ? - ? 89 2.1.1.264 additional information Smu472 does not target G2445, and this nucleotide remains unmodified in Gram-positive rRNAs. Product identification by mass spectrometry Streptococcus mutans ? - ? 89 2.1.1.264 additional information the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, reaction of EC 2.1.1.173 Staphylococcus aureus ? - ? 89 2.1.1.264 additional information the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173. Product identification by mass spectrometry Escherichia coli ? - ? 89 2.1.1.264 additional information the bifunctional methyltransferase YcbY, i.e. RlmKL, adds the m7G2069 and m2G2445 modifications in Escherichia coli 23S rRNA, recognition of dual rRNA targets by YcbY Escherichia coli BW25113 ? - ? 89 2.1.1.264 additional information the enzyme also catalyzes the N2-methylation of guanine2445 in 23S rRNA, RlmL activity, reaction of EC 2.1.1.173. Product identification by mass spectrometry Escherichia coli BW25113 ? - ? 89 2.1.1.264 S-adenosyl-L-methionine + guanine2069 in 23S rRNA - Staphylococcus aureus S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA - ? 419577 2.1.1.264 S-adenosyl-L-methionine + guanine2069 in 23S rRNA - Streptococcus mutans S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA - ? 419577 2.1.1.264 S-adenosyl-L-methionine + guanine2069 in 23S rRNA RlmK activity in helix 74 of Escherichia coli 23S rRNA Escherichia coli S-adenosyl-L-homocysteine + N7-methylguanine2069 in 23S rRNA - ? 419577