6.2.1.44 ATP + 3-(methylthio)propanoate + CoA - Ruegeria pomeroyi AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA - Candidatus Pelagibacter ubique AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA - Ruegeria lacuscaerulensis AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA - Pseudomonas aeruginosa AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA - Burkholderia thailandensis AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA degradation 3-(methylthio)propanoate, the first product in 3-(dimethylsulfonio)propanoate degradation. 3-(Dimethylsulfonio)propanoate is an osmolyte of many marine algae and certain plants. The global importance of dimethylsulfoniopropionate lies in its availability as a carbon and sulfur source for marine microorganisms and as a precursor of the gas dimethylsulfide, the oceanic emission of which leads to the formation of cloud condensation nuclei and promotion of solar radiation backscatter. Two competing pathways exist for the bacterial catabolism of 3-(dimethylsulfonio)propanoate, one releasing dimethylsulphide and the other releasing methanethiol Ruegeria pomeroyi AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Candidatus Pelagibacter ubique AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi AMP + diphosphate + 3-(methylthio)propionyl-CoA - ? 427363 6.2.1.44 ATP + 3-(methylthio)propanoate + CoA once released from phytoplankton, marine bacteria degrade dimethylsulfoniopropionate by either the cleavage pathway to form the volatile gas dimethylsulfide or the demethylation pathway, yielding methanethiol, which is readily assimilated or oxidized. The enzyme DmdB, a methylmercaptopropionate-coenzyme A ligase, catalyzes the second step in the demethylation pathway and is a major regulatory point. Two forms of DmdB are present in the marine roseobacter Ruegeria pomeroyi DSS-3, RPO_DmdB1 and RPO_DmdB2 Ruegeria pomeroyi AMP + diphosphate + 3-methylmercaptopropionyl-CoA - ? 427364 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 14% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 15% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 18% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 19% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 4% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 5% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + 3-methylbutanoate + CoA activity is 8% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + 3-methylbutanoyl-CoA - ? 428220 6.2.1.44 ATP + acetate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acetate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acetate + CoA activity is 110% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acetate + CoA activity is 151% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acetate + CoA activity is 110% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acetate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acetate + CoA activity is 151% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + acetyl-CoA - ? 12 6.2.1.44 ATP + acrylate + CoA activity is 15% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 29% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 50% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 56% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 6% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 65% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 78% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 65% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 50% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 15% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 29% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + acrylate + CoA activity is 6% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + acryloyl-CoA - ? 139 6.2.1.44 ATP + butyrate + CoA activity is 113% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 121% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 160% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 36% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 73% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 81% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 93% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 121% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 160% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 93% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 113% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + butyrate + CoA activity is 81% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + butyryl-CoA - ? 143 6.2.1.44 ATP + crotonate + CoA activity is 109% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + crotonate + CoA activity is 2% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + crotonate + CoA activity is 32% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + crotonate + CoA activity is 41% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + crotonate + CoA activity is 63% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + crotonate + CoA activity is 63% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + crotonate + CoA activity is 87% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + crotonyl-CoA - ? 188 6.2.1.44 ATP + hexanoate + CoA activity is 12% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + hexanoate + CoA activity is 17% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + hexanoate + CoA activity is 17% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + hexanoate + CoA activity is 27% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + hexanoate + CoA activity is 5% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + hexanoate + CoA activity is 62% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + hexanoate + CoA activity is 94% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + hexanoyl-CoA - ? 202 6.2.1.44 ATP + isobutyrate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + isobutyrate + CoA activity is 12% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + isobutyrate + CoA activity is 13% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + isobutyrate + CoA activity is 30% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + isobutyrate + CoA activity is 48% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + isobutyrate + CoA activity is 5% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + isobutyrate + CoA activity is 7% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + isobutyryl-CoA - ? 195 6.2.1.44 ATP + methylbutyrate + CoA activity is 10% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + methylbutyrate + CoA activity is 18% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + methylbutyrate + CoA activity is 20% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + methylbutyrate + CoA activity is 23% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + methylbutyrate + CoA activity is 24% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + methylbutyrate + CoA activity is 39% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + methylbutyrate + CoA activity is 8% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + methylbutyryl-CoA - ? 428376 6.2.1.44 ATP + pentanoate + CoA activity is 179% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 18% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 31% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 49% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 51% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 68% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 91% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 68% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + pentanoate + CoA activity is 179% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + pentanoyl-CoA - ? 144 6.2.1.44 ATP + propionate + CoA activity is 124% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 126% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 13% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 16% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 45% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 72% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 77% of the activity with 3-(methylthio)propanoate Ruegeria pomeroyi AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 124% of the activity with 3-(methylthio)propanoate Pseudomonas aeruginosa ATCC 15692 AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 13% of the activity with 3-(methylthio)propanoate Candidatus Pelagibacter ubique HTCC1062 AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 45% of the activity with 3-(methylthio)propanoate Burkholderia thailandensis ATCC 700388 AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 72% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 ATP + propionate + CoA activity is 126% of the activity with 3-(methylthio)propanoate Ruegeria lacuscaerulensis ITI-1157 AMP + diphosphate + propionyl-CoA - ? 142 6.2.1.44 additional information no activity with acetate, caprylate and caprate Ruegeria pomeroyi ? - ? 89 6.2.1.44 additional information no activity with acetate, caprylate and caprate Ruegeria lacuscaerulensis ? - ? 89 6.2.1.44 additional information no activity with acetate, caprylate and caprate Burkholderia thailandensis ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Ruegeria pomeroyi ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Candidatus Pelagibacter ubique ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Ruegeria lacuscaerulensis ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Pseudomonas aeruginosa ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Pseudomonas aeruginosa ATCC 15692 ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Candidatus Pelagibacter ubique HTCC1062 ? - ? 89 6.2.1.44 additional information no activity with acetate, caprylate and caprate Burkholderia thailandensis ATCC 700388 ? - ? 89 6.2.1.44 additional information no activity with acetate, caprylate and caprate Ruegeria lacuscaerulensis ITI-1157 ? - ? 89 6.2.1.44 additional information no activity with caprylate and caprate Ruegeria lacuscaerulensis ITI-1157 ? - ? 89