5.6.2.2 ATP + catenated DNA - Gallus gallus ADP + phosphate + decatenated DNA - ? 447017 5.6.2.2 ATP + catenated kinetoplast DNA - Homo sapiens ADP + phosphate + decatenated kinetoplast DNA - ? 447018 5.6.2.2 ATP + catenated kinetoplast DNA - Solanum lycopersicum ADP + phosphate + decatenated kinetoplast DNA - ? 447018 5.6.2.2 ATP + catenated kinetoplast DNA - Plasmodium falciparum ADP + phosphate + decatenated kinetoplast DNA - ? 447018 5.6.2.2 ATP + decatenated DNA - Homo sapiens ADP + phosphate + catenated DNA - ? 447021 5.6.2.2 ATP + decatenated DNA - Solanum lycopersicum ADP + phosphate + catenated DNA - ? 447021 5.6.2.2 ATP + DNA - Rattus norvegicus ? - ? 447022 5.6.2.2 ATP + H2O - Mycobacterium tuberculosis ADP + phosphate - ? 92967 5.6.2.2 ATP + H2O - Mycobacterium tuberculosis H37Rv ADP + phosphate - ? 92967 5.6.2.2 ATP + H2O - Mycobacterium tuberculosis ATCC 25618 ADP + phosphate - ? 92967 5.6.2.2 ATP + kinetoplast DNA - Schizosaccharomyces pombe ? - ? 447119 5.6.2.2 ATP + knotted supercoiled DNA - Homo sapiens ADP + phosphate + unknotted relaxed DNA - ? 447120 5.6.2.2 ATP + negatively supercoiled pBR322 DNA - Homo sapiens ADP + phosphate + relaxed pBR322 DNA - ? 447121 5.6.2.2 ATP + negatively supercoiled pER8:hemagglutinin plasmid DNA - Homo sapiens ADP + phosphate + relaxed pER8:hemagglutinin plasmid DNA - ? 447122 5.6.2.2 ATP + negatively supercoiled pER8:hemagglutinin plasmid DNA - Solanum lycopersicum ADP + phosphate + relaxed pER8:hemagglutinin plasmid DNA - ? 447122 5.6.2.2 ATP + negatively supercoiled pUC19 DNA - Homo sapiens ADP + phosphate + relaxed pUC19 DNA - ? 447123 5.6.2.2 ATP + relaxed pBR322 DNA - Homo sapiens ADP + phosphate + supercoiled pBR322 DNA - ? 447124 5.6.2.2 ATP + relaxed pBR322 DNA - Mycobacterium tuberculosis ADP + phosphate + supercoiled pBR322 DNA - ? 447124 5.6.2.2 ATP + relaxed pBR322 DNA - Staphylococcus aureus ADP + phosphate + supercoiled pBR322 DNA - ? 447124 5.6.2.2 ATP + relaxed pBR322 DNA - Mycobacterium tuberculosis H37Rv ADP + phosphate + supercoiled pBR322 DNA - ? 447124 5.6.2.2 ATP + relaxed pUC18 DNA enzyme that binds and hydrolyzes only one ATP undergoes nucleotide-induced N-gate closure and supercoils DNA Bacillus subtilis ADP + phosphate + supercoiled pUC18 DNA - ? 447125 5.6.2.2 ATP + supercoiled pBR322 DNA - Staphylococcus aureus ADP + phosphate + relaxed pBR322 DNA - ? 447126 5.6.2.2 ATP + supercoiled pBR322 DNA - Mycolicibacterium smegmatis ADP + phosphate + relaxed pBR322 DNA - ? 447126 5.6.2.2 ATP + supercoiled pBR322 DNA - Mycobacterium tuberculosis ADP + phosphate + relaxed pBR322 DNA - ? 447126 5.6.2.2 ATP + supercoiled pBR322 DNA - Mycobacterium tuberculosis H37Rv ADP + phosphate + relaxed pBR322 DNA - ? 447126 5.6.2.2 ATP + supercoiled pBR322 plasmid DNA - Homo sapiens ADP + phosphate + relaxed pBR322 plasmid DNA - ? 447127 5.6.2.2 ATP + supercoiled plasmid pNO1 - Homo sapiens ADP + phosphate + relaxed plasmid pNO1 - ? 447128 5.6.2.2 ATP + supercoiled pUC18 DNA - Bacillus subtilis ADP + phosphate + relaxed pUC18 DNA - ? 447129 5.6.2.2 ATP + supercoiled pUC18 plasmid - Plasmodium falciparum ADP + phosphate + relaxed pUC18 plasmid - ? 447130 5.6.2.2 ATP + supercoiled pUC19 DNA - Homo sapiens ADP + phosphate + nicked pUC19 DNA - ? 447131 5.6.2.2 ATP + supercoiled pUC19 DNA - Solanum lycopersicum ADP + phosphate + nicked pUC19 DNA - ? 447131 5.6.2.2 ATP + supercoiled pUC19 DNA - Homo sapiens ADP + phosphate + relaxed pUC19 DNA - ? 447132 5.6.2.2 ATP + supercoiled pUC19 DNA - Solanum lycopersicum ADP + phosphate + relaxed pUC19 DNA - ? 447132 5.6.2.2 catenated DNA + ATP + H2O decatenation Saccharolobus shibatae minicircular DNA + ADP + phosphate - ? 428459 5.6.2.2 catenated kDNA + ATP + H2O - Homo sapiens unlinked monomer kDNA + ADP + phosphate - ? 368826 5.6.2.2 catenated kDNA + ATP + H2O - African swine fever virus decatenated kDNA + ADP + phosphate - ? 445287 5.6.2.2 catenated kDNA + ATP + H2O very weak decatenation activity Paenibacillus polymyxa decatenated kDNA + ADP + phosphate - ? 445287 5.6.2.2 catenated kDNA + ATP + H2O very weak decatenation activity Paenibacillus polymyxa M1 decatenated kDNA + ADP + phosphate - ? 445287 5.6.2.2 closed circular DNA + ATP - Sulfolobus acidocaldarius positively supercoiled DNA + ADP + phosphate - ? 428489 5.6.2.2 closed circular DNA + ATP - Sulfolobus acidocaldarius 7 positively supercoiled DNA + ADP + phosphate - ? 428489 5.6.2.2 CVM-1 viral DNA + ATP + H2O breakage, passage, decatenation, and rejoining of CVM-1 viral DNA, viral DNA contains 10% N6-methyladenine and 42% 5-methylcytosine, 4fold decreased activity compared to unmodified DNA Chlorella virus Marburg ? - ? 379626 5.6.2.2 dATP + negatively supercoiled circular DNA - Drosophila melanogaster dADP + phosphate + relaxed circular DNA - ? 453635 5.6.2.2 dATP + negatively supercoiled circular DNA - Bos taurus dADP + phosphate + relaxed circular DNA - ? 453635 5.6.2.2 DNA the enzyme is capable of introducing positive supercoils into closed-circular DNA. It catalyzes positive supercoiling both in negatively supercoiled DNA and in relaxed DNA. The reactions require the presence of ATP Desulfurococcus amylolyticus ? - ? 376743 5.6.2.2 DNA positively supercoils DNA, the enzyme requires ATP, which is bound and hydrolysed by a helicase-like domain Archaeoglobus fulgidus positively supercoiled DNA - ? 426344 5.6.2.2 DNA + ATP decatenation, the enzyme normally catalyzes DNA transport after it hydrolyzes one ATP and before it hydrolyzes the second Saccharomyces cerevisiae ? - ? 368831 5.6.2.2 DNA + ATP cleavage requires coupling to ATP hydrolysis Homo sapiens ? - ? 368831 5.6.2.2 DNA + ATP DNA topoisomerase VI relaxes both negatively and positively supercoiled DNA in the presence of ATP and has no DNA supercoiling activity Saccharolobus shibatae ? - ? 368831 5.6.2.2 DNA + ATP + H2O - African swine fever virus DNA + ADP + phosphate - ? 424385 5.6.2.2 DNA + ATP + H2O cleavage, relaxation, decatenation, and religation of DNA Homo sapiens DNA + ADP + phosphate - ? 424385 5.6.2.2 DNA + ATP + H2O decatenation of DNA Homo sapiens DNA + ADP + phosphate - ? 424385 5.6.2.2 DNA + ATP + H2O relaxation and cleavage of DNA, cleavage requires coupling to ATP hydrolysis Saccharomyces cerevisiae DNA + ADP + phosphate - ? 424385 5.6.2.2 DNA + ATP + H2O reverse gyrase is a DNA topoisomerase endowed with ATP-dependent positive supercoiling activity. It is typical of microorganisms living at high temperature and might play a role in maintenance of genome stability and repair Saccharolobus solfataricus ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme helps disentangle chromosomes to facilitate cell division Saccharolobus shibatae ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme helps disentangle chromosomes to facilitate cell division Methanosarcina mazei ? - ? 427433 5.6.2.2 DNA + ATP + H2O binding of the enzyme on double-stranded DNA alters the DNA structure in two ways: (I) reduction of the linking number in a circular duplex after covalent closure by a ligase; (II) singe-stranded DNA cleavage. Positive supercoiling of DNA in the presence of ATP. Catalyzes ATP-dependent positive supercoiling and its stoichiometric binding to DNA Sulfolobus acidocaldarius ? - ? 427433 5.6.2.2 DNA + ATP + H2O reverse gyrase is a DNA topoisomerase endowed with ATP-dependent positive supercoiling activity Saccharolobus solfataricus ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme catalyzes an ATP-dependent positive DNA supercoiling reaction in vitro Saccharolobus shibatae ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme helps disentangle chromosomes to facilitate cell division. ATP binding elicits a major structural reorganization that is propagated to the DNA-cleavage center of the enzyme, explaining how ATP is coupled to DNA capture and strand scission Saccharolobus shibatae ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme helps disentangle chromosomes to facilitate cell division. ATP binding elicits a major structural reorganization that is propagated to the DNA-cleavage center of the enzyme, explaining how ATP is coupled to DNA capture and strand scission Methanosarcina mazei ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme helps disentangle chromosomes to facilitate cell division Methanosarcina mazei DSM 3647 ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme helps disentangle chromosomes to facilitate cell division. ATP binding elicits a major structural reorganization that is propagated to the DNA-cleavage center of the enzyme, explaining how ATP is coupled to DNA capture and strand scission Methanosarcina mazei DSM 3647 ? - ? 427433 5.6.2.2 DNA + ATP + H2O the enzyme catalyzes an ATP-dependent positive DNA supercoiling reaction in vitro Saccharolobus shibatae B12 ? - ? 427433 5.6.2.2 double-stranded DNA + ATP + H2O ATP-dependent breakage, passage, and rejoining of double-stranded DNA Homo sapiens ? - ? 424386 5.6.2.2 double-stranded DNA + ATP + H2O the enzyme generates ATP-dependent double-strand breaks with two-nucleotide overhangs on supercoiled or linear DNA. topoVI is covalently attached to the 5'-ends of the broken DNA Saccharolobus shibatae ? - ? 424386 5.6.2.2 double-stranded M13 DNA + ATP + H2O breakage, passage, and rejoining of double-stranded M13 DNA, including ATPase activity Coprinopsis cinerea ? - ? 379712 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Homo sapiens ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Mycolicibacterium smegmatis ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Paramecium bursaria Chlorella virus 1 ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA Chlorella virus Marburg ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for conrol of DNA topology Paramecium bursaria chlorella virus ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, enzyme is required for control of DNA topology Chlorella virus Marburg ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity and high strand passage activity of the viral type enzyme, tight DNA binding, mapping of DNA cleavage sites Chlorella virus Marburg ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme Paramecium bursaria Chlorella virus 1 ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme Chlorella virus Marburg ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme, mapping of DNA cleavage sites Paramecium bursaria chlorella virus ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O cleavage and religation of dsDNA Tequatrovirus T4 ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O cleavage of dsDNA, religation of dsDNA, ligation by the enzyme of the two strands of a double helix in a nonconcerted fashion, ligation of a nicked oligonucleotide in which the 5'-terminal phosphate at the nick is activated by covalent attachment to 4-nitrophenol, the active site Tyr805 is not important for catalysis of ligation Homo sapiens ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O decatenation of dsDNA Homo sapiens ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O dsDNA relaxation, breakage, decatenation, and cleavage, isozyme IIalpha relaxes positively supercoiled DNA 10times faster than negatively supercoiled DNA, isozyme IIbeta has no preference, the enzyme creates transient single- and double-stranded breaks, site-specific cleavage of supercoiled DNA Homo sapiens ? - ? 378669 5.6.2.2 dsDNA + ATP + H2O passage of dsDNA Homo sapiens ? - ? 378669 5.6.2.2 form I DNA + ATP + H2O - Rattus norvegicus form IV DNA + ADP + phosphate - ? 368821 5.6.2.2 four-way junction DNA + ATP + H2O binds and cleaves four-way junction DNA in vitro, topoisomerase IIbeta has a 4fold higher affinity for the four-way junction than for the linear duplex, the enzyme binds to the centre of the duplex, the four-way junction contains the sequence of a 40 bp linear duplex (containing a single topoisomerase cleavage site) along to adjacent arms, with the cleavage site straddling the point of strand exchange, the remaining two arms are comprised of sequences which do not contain topoisomerase cleavage sites, except that a potential m-AMSA-inducible cleavage site is introduced across the junction of these two arms Homo sapiens ? - ? 368832 5.6.2.2 kDNA + ATP + H2O decatenation Rattus norvegicus ? - ? 368830 5.6.2.2 kinetoplast DNA + ATP decatenation Homo sapiens ? - ? 368823 5.6.2.2 kinetoplast DNA + ATP + H2O breakage, passage, decatenation, and rejoining of kinetoplast DNA, kinetoplast DNA is isolated from Crithidia fasciculata Chlorella virus Marburg ? - ? 379966 5.6.2.2 kinetoplast DNA + ATP + H2O breakage, passage, decatenation, and rejoining of kinetoplast DNA, kinetoplast DNA isolated from Crithidia fasciculata, recombinant wild-type and truncation mutant Top2alphaDELTA1175, mechanisms Homo sapiens ? - ? 379966 5.6.2.2 kinetoplast DNA + ATP + H2O cleavage of kinetoplast DNA Leishmania donovani ? - ? 379966 5.6.2.2 kinetoplast DNA + ATP + H2O decatenation of kinetoplast DNA Mus musculus ? - ? 379966 5.6.2.2 kinetoplast DNA + ATP + H2O decatenation of kinetoplast DNA Homo sapiens ? - ? 379966 5.6.2.2 linear DNA fragments of viral SSV1 DNA + ATP + H2O ATP hydrolysis is necessary for DNA resealing Saccharolobus shibatae ? - ? 428771 5.6.2.2 linear DNA fragments of viral SSV1 DNA + ATP + H2O ATP hydrolysis is necessary for DNA resealing Saccharolobus shibatae B12 ? - ? 428771 5.6.2.2 additional information - Drosophila melanogaster ? - ? 89 5.6.2.2 additional information - Bacteria ? - ? 89 5.6.2.2 additional information - eukaryota ? - ? 89 5.6.2.2 additional information - Escherichia coli ? - ? 89 5.6.2.2 additional information - Rattus norvegicus ? - ? 89 5.6.2.2 additional information - Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information - Bos taurus ? - ? 89 5.6.2.2 additional information - Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information dATP can substitute for ATP Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information changes the linking number of a closed circular molecule by an increment of two Saccharolobus shibatae ? - ? 89 5.6.2.2 additional information enzyme also cleaves single-stranded DNA in a site-specific fashion, but this cleavage is mechanistically distinct from the double-strand DNA cleavage Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information changes the linking number of a closed circular molecule by integers Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information the enzyme can alter the linking number of DNA only in steps of two Drosophila melanogaster ? - ? 89 5.6.2.2 additional information the enzyme can alter the linking number of DNA only in steps of two eukaryota ? - ? 89 5.6.2.2 additional information the enzyme can alter the linking number of DNA only in steps of two Trypanosoma cruzi ? - ? 89 5.6.2.2 additional information the only topoisomerase that can introduce negative supercoils in DNA Bacteria ? - ? 89 5.6.2.2 additional information ATP hydrolysis Bacteria ? - ? 89 5.6.2.2 additional information ATP hydrolysis Xenopus laevis ? - ? 89 5.6.2.2 additional information DNA-dependent ATPase activity Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information does not catalyze DNA supercoiling Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information characterization of the interaction of topoisomerase II with DNA and identification of a DNA-binding domain, conserved DNA-binding mechanism Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information at high concentrations the enzyme can knot circular duplex DNA molecules Drosophila melanogaster ? - ? 89 5.6.2.2 additional information in contrast to DNA gyrase, eucaryotic type II topoisomerase interacts preferentially with negatively supercoiled DNA over relaxed DNA Bacteria ? - ? 89 5.6.2.2 additional information in contrast to DNA gyrase, eucaryotic type II topoisomerase interacts preferentially with negatively supercoiled DNA over relaxed DNA eukaryota ? - ? 89 5.6.2.2 additional information dATP is a poor substitute for ATP Bacteria ? - ? 89 5.6.2.2 additional information the linking number of a DNA molecule changes by one to two units for each ATP molecule hydrolyzed Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information cannot supercoil various E. coli plasmid DNAs in vitro Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information the following reactions are catalyzed in an ATP-dependent fashion: relaxation of superhelical turns, catenation, decatenation, unknotting of circular duplex DNA Drosophila melanogaster ? - ? 89 5.6.2.2 additional information the following reactions are catalyzed in an ATP-dependent fashion: relaxation of superhelical turns, catenation, decatenation, unknotting of circular duplex DNA Bacteria ? - ? 89 5.6.2.2 additional information ATP-dependent negative supercoiling of closed circular duplex DNA Escherichia coli ? - ? 89 5.6.2.2 additional information dATP can substitute for ATP. 8-Azidoadenosine 5'-triphosphate is used less efficiently by the enzyme than ATP Bos taurus ? - ? 89 5.6.2.2 additional information unknotts P4 DNA in an ATP-dependent manner Bos taurus ? - ? 89 5.6.2.2 additional information no formation of negative supercoils eukaryota ? - ? 89 5.6.2.2 additional information when large amounts of enzyme are incubated with circular duplex DNA in absence of ATP, knotted molecules of varying complexity are formed Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information unknotts knotted P4 DNA Daucus carota ? - ? 89 5.6.2.2 additional information unknotting activity Trypanosoma cruzi ? - ? 89 5.6.2.2 additional information dATP can substitute for ATP. ITP is slightly effective at 5-10 mM Mus musculus ? - ? 89 5.6.2.2 additional information in presence of a 50000 MW protein from the silk gland of Bombyx mori the enzyme introduces unconstrained negative supercoils into DNA eukaryota ? - ? 89 5.6.2.2 additional information DNA stimulated ATPase activity Bos taurus ? - ? 89 5.6.2.2 additional information characterization of the interaction between topoisomerase II and DNA by transcription footprinting Bos taurus ? - ? 89 5.6.2.2 additional information ATP-dependent generation of negative supercoils Bacteria ? - ? 89 5.6.2.2 additional information no ATPase activity Trypanosoma cruzi ? - ? 89 5.6.2.2 additional information enzyme binds Z-DNA with an affinity 2 orders of magnitude greater than that for B-DNA Drosophila melanogaster ? - ? 89 5.6.2.2 additional information dATP can substitute for ATP in the supercoiling reaction Escherichia coli ? - ? 89 5.6.2.2 additional information substrate specificity Arabidopsis thaliana ? - ? 89 5.6.2.2 additional information changes the topology of DNA by coupling binding and hydrolysis of two ATP molecules to the transport of one DNA duplex through a temporary break introduced in another Homo sapiens ? - ? 89 5.6.2.2 additional information topoisomerase I works in concert with topoisomerase II to modulate the level of DNA supercoiling eukaryota ? - ? 89 5.6.2.2 additional information the Z-DNA binding activity of undegraded topoisomerase II may be important in targeting the enzyme both to structural motifs required for chromatin organization and to sites of local supercoiling Drosophila melanogaster ? - ? 89 5.6.2.2 additional information activity is essential for kinetoplast DNA minicircle segregation Trypanosoma equiperdum ? - ? 89 5.6.2.2 additional information localization of the enzyme within the single mitochondrion suggests an important role of the enzyme in kinetoplast DNA replication Crithidia fasciculata ? - ? 89 5.6.2.2 additional information controls DNA topology by cleaving and rejoining DNA strands and passing other DNA strands through transient gaps. Plays a crucial function in the regulation of the physiological function of the genome eukaryota ? - ? 89 5.6.2.2 additional information enzyme plays a key role in DNA replication and transcription. Required for chromosome segregation during meiosis and mitosis eukaryota ? - ? 89 5.6.2.2 additional information functions in chromosome condensation and segregation Mammalia ? - ? 89 5.6.2.2 additional information isoenzyme IIalpha is expressed at the highest level during rapid proliferation, isoenzyme IIbeta is expressed maximally in cells that have reached the plateau phase of growth Homo sapiens ? - ? 89 5.6.2.2 additional information involvement in RNA polymerase II transcription eukaryota ? - ? 89 5.6.2.2 additional information possible involvement in DNA replication Rattus norvegicus ? - ? 89 5.6.2.2 additional information enzyme is required for chromosome segregation Bos taurus ? - ? 89 5.6.2.2 additional information involvement in the initiation of DNA replication Bacteria ? - ? 89 5.6.2.2 additional information biological role of topoisomerase II and DNA gyrase Bacteria ? - ? 89 5.6.2.2 additional information biological role of topoisomerase II and DNA gyrase eukaryota ? - ? 89 5.6.2.2 additional information dual role for the topoisomerase IIalpha in vivo consistent with the notion that its sequestration to the chromatin might play a role in chromosome condensation and decondesation during spermatogenesis Rattus norvegicus ? - ? 89 5.6.2.2 additional information the enzyme is essential for DNA metabolism and chromosome dynamics. It changes the topology of DNA by coupling binding and hydrolysis of two ATP molecules to the transport of one DNA duplex through a temporary break introduced in another Homo sapiens ? - ? 89 5.6.2.2 additional information DNA topoisomerase II interacts with Lim15/Dmc1 in meiosis, the enzyme suppresses Lim15/Dmc1-dependent strand transfer activity and activates its DNA-dependent ATPase activity in vitro, overview, Lim15 inhibits the topoisomerase II activity in vivo Coprinopsis cinerea ? - ? 89 5.6.2.2 additional information increased expression of the enzyme causes neoplasia Tequatrovirus T4 ? - ? 89 5.6.2.2 additional information isozyme IIbeta is no required for DNA replication Homo sapiens ? - ? 89 5.6.2.2 additional information the amount of isozyme IIalpha in cells influences directly the cell cycle kinetics in G2 and early mitosis as well as the resistance to nocodazole Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme induces the activity of psorospermin, O5-methyl-psorospermin, and gemcitabine alkylating DNA through an epoxide-mediated electrophilic attack, at some DNA sites leading to increased antitumor activity of the alkylating reagents Mus musculus ? - ? 89 5.6.2.2 additional information the enzyme is essential for controlling the conformation of both DNA and whole chromosomes, e.g. for shortening of the chromosome axes, isozyme IIbeta can partially substitute for isozyme IIalpha Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme is essential for genomic integrity Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme is important in chromosome segregation Homo sapiens ? - ? 89 5.6.2.2 additional information activity is measured using the decatenation assay with kinetoplast DNA as a substrate Homo sapiens ? - ? 89 5.6.2.2 additional information ATPase activity is shown by the wild-type enzyme and the truncation mutant comprising residues 1-1058, the enzyme changes the DNA topology by coupling ATP hydrolysis to the transport of one DNA helix through a transient double-stranded break in another Leishmania donovani ? - ? 89 5.6.2.2 additional information cleavage of supercoiled pBR322 DNA Schizosaccharomyces pombe ? - ? 89 5.6.2.2 additional information enzyme shows ATPase activity Rattus norvegicus ? - ? 89 5.6.2.2 additional information formation of a covalent enzyme-DNA complex is a prerequisite for activity, the intermediate can destabilize the genome Chlorella virus Marburg ? - ? 89 5.6.2.2 additional information formation of a covalent enzyme-DNA complex is a prerequisite for activity, the intermediate can destabilize the genome Paramecium bursaria chlorella virus ? - ? 89 5.6.2.2 additional information isozyme IIalpha has the potential to alleviate torsional stress ahead of replication forks in an efficient and safe manner Homo sapiens ? - ? 89 5.6.2.2 additional information isozyme IIalpha interacts with the microtubule-directed agent nocodazole, overview Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent Paramecium bursaria Chlorella virus 1 ? - ? 89 5.6.2.2 additional information the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent Chlorella virus Marburg ? - ? 89 5.6.2.2 additional information the enzyme enhances the activity of psorospermin, O5-methyl-psorospermin, and gemcitabine, alkylating DNA through an epoxide-mediated electrophilic attack, at some DNA sites, overview Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme has intrinsic ATPase activity Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme introduces positively supercoils in both relaxed and negatively supercoiled DNA in an ATP-dependent manner Mycolicibacterium smegmatis ? - ? 89 5.6.2.2 additional information the enzyme performs ATP hydrolysis activity Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme performs ATP hydrolysis activity Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information the enzyme reaction is modulated by the C-terminus of the enzyme, which is also responsible for sensitivity to anti-cancer drugs Homo sapiens ? - ? 89 5.6.2.2 additional information role of enzyme in activation/repression of developmentally regulated genes at late stages of neuronal differentiation Mus musculus ? - ? 89 5.6.2.2 additional information topoisomerase II relaxes nucleosomal DNA much faster than topoisomerase I, and the DNA cross-inversion mechanism of topomerase II is facilitated in chromatin. Enzyme is the main modulator of DNA topology in chromatin fibers Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information enzyme is able to discern the handedness of supercoils during the cleavage and preferentially cut negatively supercoiled DNA. Bimodal recognition of DNA geometry in which topoisomerase II uses elements in the C-terminal domain to sense the handedness of supercoils during DNA relaxation and elements in the conserved N-terminal domain or central domain during DNA cleavage Paramecium bursaria Chlorella virus-1 ? - ? 89 5.6.2.2 additional information enzyme is able to discern the handedness of supercoils during the cleavage and preferentially cut negatively supercoiled DNA. Bimodal recognition of DNA geometry in which topoisomerase II uses elements in the C-terminal domain to sense the handedness of supercoils during DNA relaxation and elements in the conserved N-terminal domain or central domain during DNA cleavage Paramecium bursaria Chlorella virus Marburg 1 ? - ? 89 5.6.2.2 additional information G-DNA binds with higher affinity than T-DNA. enzyme with only G-DNA bound is competent to cleave DNA and the ATPase activity of enzyme solely bound to G-DNA is partially stimulated. Full stimulation requires binding of T-DNA Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information the sequence that defines a cleavage site resides within the central 20 bp of a dNA duplex. The DNA affinity does not correlate with the ability of the enzyme to cleave DNA. The binding step does not contribute significantly to the selection mechanism Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information indirect involvement of topoisomerase II alpha in the formation of radiation-induced chromatid breaks from DNA double-strand breaks, topoisomerase II alpha is a possible factor in the inter-individual variation in chromatid radiosensitivity Homo sapiens ? - ? 89 5.6.2.2 additional information mitochondrial topoisomerase II maintains kinetoplast DNA network topology by constantly remodeling the network during replication to maintain a proper minicircle density and network structure Trypanosoma brucei ? - ? 89 5.6.2.2 additional information RNA binding influences the catalytic function of topoisomerase IIalpha to regulate DNA topology, topoisomerase II sequentially interacts with the (G/U)-rich region and poly(A)+ tail of the transcript to regulate the template DNA topology in coordination with transcription termination and poly(A)+ RNA export Homo sapiens ? - ? 89 5.6.2.2 additional information Top2 plays a role in centromeric chromatin compaction Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information TopoIIalpha is essential for chromosome segregation performing the DNA surgery necessary to decatenate replicated chromosomes Mus musculus ? - ? 89 5.6.2.2 additional information topoisomerase II ensures proper sister chromatid separation through a direct role in centromere resolution and prevents incorrect microtubule-kinetochore attachments by allowing proper activation of Aurora B kinase, TOPO II activity is required to establish amphitelic kinetochore attachment, TOPO II is required for centromere separation independently of the cohesin complex Drosophila melanogaster ? - ? 89 5.6.2.2 additional information topoisomerase IIalpha is a multifunctional enzyme that catalyzes the relaxation of supercoiled DNA, decatenation of interlinked DNA and unknotting of intramolecularly linked DNA by passing a DNA helix through a transient double-strand break in a second helix Homo sapiens ? - ? 89 5.6.2.2 additional information human cells can rapidly activate and deactivate DNA topoisomerase II in response to a signal molecule, the activity of DNA topoisomerase I is highly regulated in HuT 78 cells upon treatment with interleukin-2, overview Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme introduces transient double strand breaks to alter DNA topology, generation of a transient double strand break, with each subunit breaking one DNA strand. The mechanism of DNA cleavage provides several distinct advantages including the protection of DNA ends and the ability to quickly and efficiently religate the DNA strand break. The positively supercoiled DNA at the replication fork can isomerize by migration of the positive supercoiling into wrapping of the two replicated strands. This structure called a precatenane, is a substrate for Top2 mediated DNA catenation, and may represent a plausible mechanism for Top2 action during replication elongation Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme introduces transient double strand breaks to alter DNA topology, generation of a transient double strand break, with each subunit breaking one DNA strand. The mechanism of DNA cleavage provides several distinct advantages including the protection of DNA ends and the ability to quickly and efficiently religate the DNA strand break. The positively supercoiled DNA at the replication fork can isomerize by migration of the positive supercoiling into wrapping of the two replicated strands. This structure called a precatenane, is a substrate for Top2 mediated DNA catenation, and may represent a plausible mechanism for Top2 action during replication elongation Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information the top2-191 allele acts in a dominant manner to suppress mutant taz1DELTA cold sensitivity. This suppression does not rely on the decatenation activity of Top2 and is independent of its DNA strand-passage activity. Taz1 binds telomeres, which promotes smooth replication fork progression through the repetitive telomeric sequences. The enhanced presence of reaction intermediates in which Top2 is clamped around DNA, promotes the removal of telomeric entanglements in vivo, independently of catalytic cycle completion. Modelling of how the clamped enzyme-DNA complex promotes proper chromosomal segregation, overview Schizosaccharomyces pombe ? - ? 89 5.6.2.2 additional information top2a is required for decatenation but not for condensation in embryonic mitoses Danio rerio ? - ? 89 5.6.2.2 additional information catalytic cycle of topoisomerase II, overview Homo sapiens ? - ? 89 5.6.2.2 additional information decatenation assay with substrate kinetoplast DNA from protozoa Crithidia fasciculata, and DNA relaxation assay using pRYG plasmid DNA as substrate Homo sapiens ? - ? 89 5.6.2.2 additional information DNA cleavage, aperture, closure and religation are critical steps in the topo II reaction cycle, topo II DNA gate dynamics, single-molecule FRET experiments, role of T-segment in gate opening, overview Homo sapiens ? - ? 89 5.6.2.2 additional information DNA cleavage, aperture, closure and religation are critical steps in the topo II reaction cycle, topo II DNA gate dynamics, single-molecule FRET experiments, role of T-segment in gate opening, overview Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information DNA intercalating, relaxation, and decantenation of supercoiled pBR322 DNA Homo sapiens ? - ? 89 5.6.2.2 additional information reaction mechanism, DNA cleavage by Top2 uses a tyrosine that is activated to attack the phosphodiester backbone of DNA and form a phosphotyrosine linkage, overview Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information reaction mechanism, DNA cleavage by Top2 uses a tyrosine that is activated to attack the phosphodiester backbone of DNA and form a phosphotyrosine linkage. Topological changes in DNA require the introduction of DNA strand breaks, and topoisomerases provide a safe mechanism for introducing these changes, overview Homo sapiens ? - ? 89 5.6.2.2 additional information structure-activity relationship study, overview Homo sapiens ? - ? 89 5.6.2.2 additional information structure-activity relationship, structure modelling and modelling of ligand docking in the ATP binding pocket, binding mechanism, overview Homo sapiens ? - ? 89 5.6.2.2 additional information substrate is kinetoplast DNA for decantenation by TOPOIIalpha Homo sapiens ? - ? 89 5.6.2.2 additional information substrate is pBR322 supercoiled DNA Homo sapiens ? - ? 89 5.6.2.2 additional information substrate is supercoiled pBR322 DNA Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme converts kinetoplast DNA to the decatenated form. Cleavage of pBR322 DNA Cricetulus griseus ? - ? 89 5.6.2.2 additional information topoisomerase II modulates DNA topology by generating double-stranded breaks in DNA. The presence of a nick at one scissile bond dramatically increases the rate of cleavage by human topoisomerase IIalpha at the scissile bond on the opposite strand, mechanism, overview Homo sapiens ? - ? 89 5.6.2.2 additional information the C-terminal domain of topoisomerase IIbeta but not topoisomerase IIalpha affects the binding of the enzyme to the DNA. It alters the enzyme's KD for DNA binding. Differential modes of action of the two isoforms in vivo, overview Homo sapiens ? - ? 89 5.6.2.2 additional information Topo II and Mod(mdg4)2.2 proteins directly interact Drosophila melanogaster ? - ? 89 5.6.2.2 additional information topoisomerase II is an enzyme that alters the topological state of DNA via a transient covalent enzyme-bridged double strand break in the DNA, through which a second DNA helix can pass Mus musculus ? - ? 89 5.6.2.2 additional information cleavage of negatively supercoiled pBR322 plasmid DNA and of pRYG supercoiled DNA by topoisomerase IIalpha Homo sapiens ? - ? 89 5.6.2.2 additional information cleavage of negatively supercoiled pBR322 plasmid DNA by topoisomerase IIalpha Homo sapiens ? - ? 89 5.6.2.2 additional information for DNA cleavage, the correct positioning of the catalytic tyrosine on the CAP domain with respect to the topoisomerase/primase domain seems to be achieved by a rigid body-domain movement, catalytic mechanism and role of the metal-binding TOPRIM domains in catalysis, overview Staphylococcus aureus ? - ? 89 5.6.2.2 additional information isozyme topo IIbeta substrate is plasmid DNA Rattus norvegicus ? - ? 89 5.6.2.2 additional information kinetoplast DNA is used as a substrate in a decatenation assay Homo sapiens ? - ? 89 5.6.2.2 additional information substrate for DNA topo IIalpha is supercoiled pBR322 plasmid DNA Homo sapiens ? - ? 89 5.6.2.2 additional information substrate for topo IIalpha is supercoiled pBR322 plasmid DNA. Topo II-mediated DNA strand passage requires ATP binding, so reactions in the absence of ATP represent the cleavage and relegation events that take place prior to topo II catalyzed DNA strand passage Homo sapiens ? - ? 89 5.6.2.2 additional information substrate for topo IIbeta is kinetoplast DNA. Topoisomerase II catalyzes topological changes in DNA and decatenation reaction. Residue Tyr656 of topoisomerase IIbeta is important for its catalytic activity, overview Homo sapiens ? - ? 89 5.6.2.2 additional information substrate is negatively supercoiled pBR322 plasmid DNA Homo sapiens ? - ? 89 5.6.2.2 additional information substrate is plasmid DNA Homo sapiens ? - ? 89 5.6.2.2 additional information substrate is supercoiled plasmid DNA Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme contains an active site tyrosine within the winged helix domain, role of the C-terminal domain in the topoisomerase II-DNA interaction, overview Homo sapiens ? - ? 89 5.6.2.2 additional information transport of the T-segment through the transiently cleaved G-DNA segment and the opened C-gate is via a free diffusion mechanism, opening and closing dynamics of the C-gate, two-gate model for chemomechanical coupling of topoisomerase II, detailed overview Homo sapiens ? - ? 89 5.6.2.2 additional information decatenation assay with kinetoplast DNA, DNA strand break analysis using supercoiled plasmid DNA pBR322 or pRYG Homo sapiens ? - ? 89 5.6.2.2 additional information DNA strand breakage formation in vitro assay using RF-f1p supercoiled DNA Homo sapiens ? - ? 89 5.6.2.2 additional information helicase activity for both the N-terminal domain and the full-length reverse gyrase Sulfolobus acidocaldarius ? - ? 89 5.6.2.2 additional information reconstitution of the heterodimeric active reverse gyrase from the two recombinant proteins overexpressed in Escherichia coli and purification. Subunit RgyB has a DNA-dependent ATPase activity at high temperature (80 °C) and is independent of the presence of subunit RgyA. Subunit RgyA alone has no detectable activity. The addition of subunit RgyA to subunit RgyB reconstitutes positive supercoiling activity Methanopyrus kandleri ? - ? 89 5.6.2.2 additional information substrate is negatively-supercoiled plasmid DNA Saccharomyces cerevisiae ? - ? 89 5.6.2.2 additional information the enzyme also catalyzes ATP-dependent unwinding of synthetic Holliday junctions and ATP-stimulated annealing of unconstrained single-stranded oligonucleotides Pyrobaculum calidifontis ? - ? 89 5.6.2.2 additional information the enzyme induces positive supercoiling into DNA molecules in an ATP-dependent reaction. The isolated ATPase and topoisomerase domains of reverse gyrase form specific physical interactions, retain their own DNA binding and enzymatic activities, and when combined cooperate to achieve the unique ATP-dependent positive supercoiling activity Saccharolobus solfataricus ? - ? 89 5.6.2.2 additional information TopoIIalpha-mediated catenation of plasmid DNA and kinetoplast DNA comprising small interlocked supercoiled circular DNA Homo sapiens ? - ? 89 5.6.2.2 additional information topoisomerase II action on supercoiled DNA Homo sapiens ? - ? 89 5.6.2.2 additional information the enzyme cannot supercoil relaxed DNA African swine fever virus ? - ? 89 5.6.2.2 additional information the enzyme creates ATP-dependent transient double strand breaks in a segment of DNA, passage of un-broken segment of DNA though these transient breaks followed by re-ligation of broken-ends Homo sapiens ? - ? 89 5.6.2.2 additional information negative DNA supercoiling activity assays using relaxed pBR322 DNA as substrate, and ATPase assays Mycobacterium tuberculosis ? - - 89 5.6.2.2 additional information the enzyme induces positive supercoiling into DNA molecules in an ATP-dependent reaction. The isolated ATPase and topoisomerase domains of reverse gyrase form specific physical interactions, retain their own DNA binding and enzymatic activities, and when combined cooperate to achieve the unique ATP-dependent positive supercoiling activity Saccharolobus solfataricus P2 ? - ? 89 5.6.2.2 additional information negative DNA supercoiling activity assays using relaxed pBR322 DNA as substrate, and ATPase assays Mycobacterium tuberculosis H37Rv ? - - 89 5.6.2.2 additional information negative DNA supercoiling activity assays using relaxed pBR322 DNA as substrate, and ATPase assays Mycobacterium tuberculosis ATCC 25618 ? - - 89 5.6.2.2 additional information top2a is required for decatenation but not for condensation in embryonic mitoses Danio rerio AB ? - ? 89 5.6.2.2 negatively supercoiled DNA the enzyme catalyzes a ATP-dependent DNA-positive supercoiling reaction of closed DNA plasmids. ATP is required for a correct coordination of DNA cleavage. The enzyme is able to induce positive supercoiling with ATP concentrations of 0.0001 mM. The efficiency of the reaction increases with increasing nucleotide concentrations, with an optimum between 0.1 and 1.0 mM. In the absence of ATP, the enzyme shows weak type I topoisomerase-like DNA relaxation activity. At all temperatures, relaxed and/or positive topoisomers are produced when a certain amount of the negative substrate is still present, thus suggesting that the enzyme is highly processive, i.e. it performs multiple supercoiling cycles before detaching from DNA and attacking a new substrate molecule. In the absence of ATP the enzyme shows weak type I topoisomerase-like DNA relaxation activity Pyrobaculum calidifontis positively supercoiled DNA - ? 428834 5.6.2.2 negatively supercoiled DNA + ATP + H2O relaxation Homo sapiens ? - ? 368822 5.6.2.2 negatively supercoiled DNA + ATP + H2O relaxation Bos taurus ? - ? 368822 5.6.2.2 negatively supercoiled DNA + ATP + H2O ATP-dependent relaxation of negatively supercoiled DNA Mycolicibacterium smegmatis ? - ? 368822 5.6.2.2 negatively supercoiled DNA + ATP + H2O activity of TopR2 of is strictly dependent on the presence of ATP. The enzyme exhibits an high intrinsic processivity. It is able to introduce a very high number of positive superturns in DNA Saccharolobus solfataricus highly positively supercolied DNA + ADP + phosphate - ? 428832 5.6.2.2 negatively supercoiled DNA + ATP + H2O - Methanopyrus kandleri positively supercoiled DNA + ADP + phosphate - ? 428833 5.6.2.2 negatively supercoiled DNA + ATP + H2O the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA Sulfolobus acidocaldarius positively supercoiled DNA + ADP + phosphate - ? 428833 5.6.2.2 negatively supercoiled DNA + ATP + H2O - Saccharolobus solfataricus positively supercoil DNA + ADP + phosphate - ? 429352 5.6.2.2 negatively supercoiled DNA + ATP + H2O TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells Saccharolobus solfataricus positively supercoil DNA + ADP + phosphate - ? 429352 5.6.2.2 negatively supercoiled DNA + ATP + H2O - Saccharolobus solfataricus P2 positively supercoil DNA + ADP + phosphate - ? 429352 5.6.2.2 negatively supercoiled DNA + ATP + H2O TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells Saccharolobus solfataricus P2 positively supercoil DNA + ADP + phosphate - ? 429352 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent activity. The same enzyme shows both relaxation of negatively supercoiled DNA and positive supercoiling activity Sulfolobus acidocaldarius ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Pyrococcus furiosus ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Sulfolobus sp. ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Methanothermus fervidus ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Archaeoglobus fulgidus ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Methanopyrus kandleri ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Thermococcus sp. ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Pyrodictium occultum ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Pyrobaculum neutrophilum ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Pyrobaculum islandicum ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Acidianus infernus ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Thermococcus celer ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Desulfurococcus saccharovorans ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Sulfolobus sp. B12 ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA ATP-dependent positive supercoiling activity Thermococcus sp. OB9 ? - ? 428835 5.6.2.2 negatively supercoiled pBR322 DNA + ATP + H2O breakage, passage, and rejoining of negatively supercoiled pBR322 DNA Paramecium bursaria chlorella virus ? - ? 380130 5.6.2.2 negatively supercoiled pBR322 DNA + ATP + H2O breakage, passage, decatenation, and rejoining of negatively supercoiled pBR322 DNA Chlorella virus Marburg ? - ? 380130 5.6.2.2 negatively supercoiled pBR322 DNA + ATP + H2O breakage, passage, decatenation, and rejoining of negatively supercoiled pBR322 DNA, recombinant wild-type and truncation mutant Top2alphaDELTA1175, mechanisms Homo sapiens ? - ? 380130 5.6.2.2 negatively supercoiled pHOTI plasmid DNA + ATP + H2O - Homo sapiens ? - ? 424390 5.6.2.2 negatively supercoiled plasmid DNA + ATP + H2O the full-length recombinant enzyme sustains ATP-dependent positive supercoiling. The C-terminal half of Sulfolobus reverse gyrase, expressed in Escherichia coli, exhibits a topoisomerase I activity, independent of the presence of ATP and specific of negative supercoils.The N-terminal domain does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, reminiscent of a protein motor Sulfolobus acidocaldarius negatively supercoiled plasmid DNA + ADP + phosphate - ? 428836 5.6.2.2 negatively supercoiled plasmid pBR322 DNA + ATP + H2O - Paenibacillus polymyxa relaxed plasmid pBR322 DNA + ADP + phosphate - ? 445748 5.6.2.2 negatively supercoiled plasmid pBR322 DNA + ATP + H2O - Paenibacillus polymyxa M1 relaxed plasmid pBR322 DNA + ADP + phosphate - ? 445748 5.6.2.2 negatively supercoiled pUC18 plasmid DNA + ATP + H2O - Rattus norvegicus relaxed pUC18 plasmid DNA + ADP + phosphate - ? 445749 5.6.2.2 network of DNA rings + ATP + H2O decatenation of kDNA networks Trypanosoma cruzi monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation of kDNA networks Trypanosoma equiperdum monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Cricetulus griseus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Drosophila melanogaster monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Mammalia monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Bacteria monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Staphylococcus aureus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation eukaryota monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Bacillus subtilis monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Mus musculus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Escherichia coli monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Homo sapiens monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Rattus norvegicus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Saccharomyces cerevisiae monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Bos taurus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Pisum sativum monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Pseudomonas aeruginosa monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Crithidia fasciculata monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Xenopus laevis monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Schizosaccharomyces pombe monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Micrococcus luteus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Trypanosoma cruzi monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Daucus carota monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Tequatrovirus T4 monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Citrobacter freundii monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Brassica oleracea monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Trypanosoma equiperdum monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Saccharolobus shibatae monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Streptomyces niveus monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Sulfolobus sp. monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O enzyme is more efficient in decatenation than relaxation Saccharolobus shibatae monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation of Trypanosoma cruzi kDNA Daucus carota monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O in absence of an DNA-binding protein: decatenation and unknotting of the DNA rings Saccharomyces cerevisiae monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O decatenation Sulfolobus sp. B12 monomeric DNA circles + ADP + phosphate - ? 1297 5.6.2.2 network of DNA rings + ATP + H2O - Trypanosoma brucei monomer DNA circles + ADP + phosphate - ? 401527 5.6.2.2 PBCV-1 viral DNA + ATP + H2O breakage, passage, decatenation, and rejoining of PBCV-1 viral DNA, viral DNA contains N6-methyladenine and 5-methylcytosine, decreased activity compared to unmodified DNA Paramecium bursaria Chlorella virus 1 ? - ? 380188 5.6.2.2 pBR322 DNA + ATP + H2O cleavage of pBR322 DNA Rattus norvegicus ? - ? 380189 5.6.2.2 pBR322 DNA + ATP + H2O decatenation, relaxation and cleavage of pBR322 DNA Homo sapiens ? - ? 380189 5.6.2.2 plasmid DNA + ATP + H2O cleavage of plasmid DNA Homo sapiens ? - ? 380211 5.6.2.2 plasmid DNA pRYG + ATP + H2O cleavage of plasmid DNA pRYG Homo sapiens ? - ? 380210 5.6.2.2 positively supercoiled plasmid pBR322 DNA + ATP + H2O - Paenibacillus polymyxa relaxed plasmid pBR322 DNA + ADP + phosphate - ? 445829 5.6.2.2 positively supercoiled plasmid pBR322 DNA + ATP + H2O - Paenibacillus polymyxa M1 relaxed plasmid pBR322 DNA + ADP + phosphate - ? 445829 5.6.2.2 relaxed DNA + ATP + H2O - Methanopyrus kandleri positively supercoiled DNA + ADP + phosphate - ? 428911 5.6.2.2 relaxed DNA + ATP + H2O the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA Sulfolobus acidocaldarius positively supercoiled DNA + ADP + phosphate - ? 428911 5.6.2.2 relaxed pBR322 plasmid + ATP + H2O supercoiling Saccharolobus shibatae supercoiled pBR322 plasmid + ADP + phosphate - ? 428912 5.6.2.2 supercoiled DNA + ATP - Homo sapiens relaxed DNA + ADP + phosphate - ? 444406 5.6.2.2 supercoiled DNA + ATP + H2O - Cricetulus griseus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Drosophila melanogaster catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Staphylococcus aureus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Bacillus subtilis catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Mus musculus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Escherichia coli catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Homo sapiens catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Saccharomyces cerevisiae catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Bos taurus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Crithidia fasciculata catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Micrococcus luteus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Coprinopsis cinerea catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Candida albicans catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Plasmodium berghei catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Leishmania donovani catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Cricetulus griseus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Drosophila melanogaster catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Mammalia catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Bacteria catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Staphylococcus aureus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation eukaryota catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Bacillus subtilis catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Mus musculus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Escherichia coli catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Homo sapiens catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Rattus norvegicus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Saccharomyces cerevisiae catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Bos taurus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Pisum sativum catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Pseudomonas aeruginosa catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Crithidia fasciculata catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Xenopus laevis catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Schizosaccharomyces pombe catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Micrococcus luteus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Trypanosoma cruzi catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Daucus carota catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Tequatrovirus T4 catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Citrobacter freundii catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Brassica oleracea catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Trypanosoma equiperdum catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Saccharolobus shibatae catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Streptomyces niveus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Sulfolobus sp. catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Xenopus laevis catenated DNA networks + ADP + phosphate in presence of spermine circular DNA is catenated to dimers, trimer and a network ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Saccharomyces cerevisiae catenated DNA networks + ADP + phosphate network of DNA rings that are topologically interlocked ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O in presence of a yeast DNA-binding protein Saccharomyces cerevisiae catenated DNA networks + ADP + phosphate network of DNA rings that are topologically interlocked ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation in presence of the condensing agent histone H1 Drosophila melanogaster catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O in presence of condensing agents like histone H1 or spermidine Bos taurus catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation in absence of ATP Trypanosoma cruzi catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O covalently closed double-stranded DNA rings Saccharomyces cerevisiae catenated DNA networks + ADP + phosphate network of DNA rings that are topologically interlocked ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O catenation Sulfolobus sp. B12 catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Leishmania donovani MHOM/80/IN/Dd8 catenated DNA networks + ADP + phosphate - ? 1296 5.6.2.2 supercoiled DNA + ATP + H2O - Crithidia fasciculata relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O - Homo sapiens relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Cricetulus griseus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Drosophila melanogaster relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Mammalia relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Bacteria relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Staphylococcus aureus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation eukaryota relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Bacillus subtilis relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Mus musculus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Escherichia coli relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Homo sapiens relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Rattus norvegicus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Saccharomyces cerevisiae relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Bos taurus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Pisum sativum relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Pseudomonas aeruginosa relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Crithidia fasciculata relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Xenopus laevis relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Schizosaccharomyces pombe relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Micrococcus luteus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Trypanosoma cruzi relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Daucus carota relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Tequatrovirus T4 relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Citrobacter freundii relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Brassica oleracea relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Trypanosoma equiperdum relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Saccharolobus shibatae relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Streptomyces niveus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Sulfolobus sp. relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation Rattus norvegicus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation Crithidia fasciculata relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation in absence of ATP Bacteria relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation of supercoiled pBR322 DNA Homo sapiens relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation of negative and positive supercoils eukaryota relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation of negative and positive supercoils Saccharolobus shibatae relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation of negative and positive supercoils Sulfolobus sp. relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Bacteria relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA eukaryota relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Escherichia coli relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Saccharomyces cerevisiae relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Bos taurus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively or positively supercoiled DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O pBR322 plasmid DNA Tequatrovirus T4 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O dATP can substitute for ATP Bos taurus relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O under optimal conditions the enzyme relaxes all supercoils prior to dissociation of the enzyme from DNA eukaryota relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O positive supercoils are relaxed in presence of beta,gamma-imido ATP Bacteria relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation of negatively supercoiled DNA Saccharomyces cerevisiae relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O under optimal conditions relaxation of negatively supercoiled DNA occurs in a highly processive manner eukaryota relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O DNA cleavage by topo VI generates two-nucleotide 5'-protruding ends Saccharolobus shibatae relaxed DNA + ADP + phosphate - ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O relaxation Sulfolobus sp. B12 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O ATP-dependent relaxation of negative and positive supercoils Sulfolobus sp. B12 relaxed DNA + ADP + phosphate ADP is cleaved to ADP and phosphate ? 1298 5.6.2.2 supercoiled DNA + ATP + H2O substrate is supercoiled scpBR322 plasmid DNA. Topo2a creates transient breaks in supercoiled DNA in an ATP-dependent manner resulting in DNA relaxation Homo sapiens ? - ? 424389 5.6.2.2 supercoiled dsDNA relaxation of supercoiled dsDNA, reaction mechanism Arabidopsis thaliana ? - ? 380337 5.6.2.2 supercoiled pBR 322 plasmid DNA + ATP + H2O - Homo sapiens relaxed pBR 322 plasmid DNA + ADP + phosphate - ? 401986 5.6.2.2 supercoiled pBR322 + ATP relaxation Homo sapiens ? - ? 368828 5.6.2.2 supercoiled pBR322 DNA cleavage of supercoiled pBR322 DNA Homo sapiens ? - ? 380338 5.6.2.2 supercoiled pBR322 DNA + ATP relaxation Rattus norvegicus ? - ? 368829 5.6.2.2 supercoiled pBR322 DNA + ATP + H2O - Homo sapiens relaxed pBR322 DNA + ADP + phosphate - ? 444408 5.6.2.2 supercoiled pBR322 plasmid + ATP + H2O relaxation Saccharolobus shibatae relaxed pBR322 plasmid + ADP + phosphate - ? 428960 5.6.2.2 supercoiled pBR322 plasmid DNA + ATP + H2O - African swine fever virus relaxed pBR322 plasmid DNA + ADP + phosphate - ir 445890 5.6.2.2 supercoiled pKMp27 DNA + ATP + H2O relaxation of supercoiled pKMp27 DNA Homo sapiens ? - ? 380339 5.6.2.2 supercoiled plasmid DNA + ATP + H2O - Homo sapiens relaxed DNA + ADP + phosphate - ? 368824 5.6.2.2 supercoiled plasmid pBR322 DNA + ATP relaxation of supercoiled DNA Homo sapiens ? - ? 368827 5.6.2.2 supercoiled pRYG DNA + ATP + H2O cleavage of supercoiled pRYG DNA Homo sapiens ? - ? 380340 5.6.2.2 supercoiled pRYG DNA + ATP + H2O relaxation and cleavage of supercoiled pRYG DNA Homo sapiens ? - ? 380340 5.6.2.2 supercoiled pRYG DNA + ATP + H2O relaxation of supercoiled pRYG DNA Rattus norvegicus ? - ? 380340 5.6.2.2 supercoiled pRYG DNA + ATP + H2O relaxation of supercoiled pRYG DNA Leishmania donovani ? - ? 380340 5.6.2.2 theta-174 phage DNA + ATP + H2O unknotting of theta-174 phage DNA Homo sapiens ? - ? 380344 5.6.2.2 topoligically relaxed plasmid DNA + ATP catenation in presence of a DNA crowding agent Bos taurus ? - ? 368825