4.6.1.24 5'-OHGGGACAGUAUUUG-3' + H2O - Escherichia coli ? - ? 452633 4.6.1.24 5'-pGGGACAGUAUUUG-3' + H2O - Escherichia coli ? - ? 452634 4.6.1.24 adenylyl-3',5'-cytidine ApC Aspergillus oryzae adenosine-2',3'-cyclic phosphate + cytidine - ? 372266 4.6.1.24 adenylyl-3',5'-cytidine + H2O kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W Aspergillus oryzae adenosine 3'-phosphate + cytidine - ? 139778 4.6.1.24 adenylyl-3',5'-cytidine + H2O kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) Aspergillus oryzae adenosine 3'-phosphate + cytidine - ? 139778 4.6.1.24 ApC + H2O - Aspergillus oryzae adenosine 3'-phosphate + cytidine - ? 372265 4.6.1.24 GpA + H2O - Aspergillus oryzae guanosine 3'-phosphate + adenosine - ? 452635 4.6.1.24 GpC + H2O - Aspergillus oryzae guanosine 3'-phosphate + cytidine - ? 373359 4.6.1.24 GpC + H2O - Ustilago sphaerogena guanosine 3'-phosphate + cytidine - ? 373359 4.6.1.24 GpC + H2O enzyme-catalyzed hydrolysis of GpC Aspergillus oryzae ? - ? 418702 4.6.1.24 GpG + H2O - Aspergillus oryzae guanosine 3'-phosphate + guanosine - ? 452636 4.6.1.24 GpU + H2O - Aspergillus oryzae guanosine 3'-phosphate + uridine - ? 373358 4.6.1.24 GpU + H2O hydrolysis Aspergillus oryzae guanosine 3'-phosphate + uridine - ? 373358 4.6.1.24 guanosine 2',3'-cyclic phosphate + H2O - Aspergillus oryzae guanosine-3'-phosphate - ? 12776 4.6.1.24 guanosine 2',3'-cyclic phosphate + H2O Ustilago sphaerogena, RNase U1 Ustilago sphaerogena guanosine-3'-phosphate - ? 12776 4.6.1.24 guanosine-2',3'-cyclic phosphate + H2O hydrolysis Aspergillus oryzae guanosine 3'-phosphate - ? 373357 4.6.1.24 guanylyl(3',5') adenosine + H2O shift in nucleotide conformational equilibrium contributes to increased rate of catalysis of guanylyl(3',5') adenosine 3'-monophosphate versus guanylyl(3',5') adenosine Bacillus amyloliquefaciens ? - ? 382013 4.6.1.24 guanylyl(3',5') adenosine 3'-monophosphate + H2O shift in nucleotide conformational equilibrium contributes to increased rate of catalysis of guanylyl(3',5') adenosine 3'-monophosphate versus guanylyl(3',5') adenosine Bacillus amyloliquefaciens ? - ? 382014 4.6.1.24 guanylyl-3',5'-cytidine Transphosphorylation reaction Aspergillus oryzae guanosine-2',3'-cyclic phosphate + cytidine - ? 373361 4.6.1.24 guanylyl-3',5'-cytidine + H2O GpC Aspergillus oryzae guanosine 3'-phosphate + cytidine - ? 373363 4.6.1.24 guanylyl-3',5'-cytidine + H2O kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, 195 for mutant enzyme E46N and 137 for mutant enzyme E46N/Y45W Aspergillus oryzae ? - ? 382015 4.6.1.24 guanylyl-3',5'-cytidine + H2O kcat/Km(GpC)/kcat/Km(ApC) is 284000 for wild-type enzyme, and 39 for mutant enzyme K41E/Y42F/N43R/Y45W/E46N/W59Y (RNase T1-R2) and mutant enzyme K41E/Y42F/N43R/Y45W/E46N (RNase RV) Aspergillus oryzae ? - ? 382015 4.6.1.24 guanylyl-3',5'-uridine Transphosphorylation reaction with GpU or the diastereomers resulting from thio-substitution of a nonbridging oxygen of GpU, RpGp(S)U and SpGp(S)U as substrates. SpGp(S)U is a very poor substrate for wild type enzyme Aspergillus oryzae guanosine-2',3'-cyclic phosphate + uridine - ? 373362 4.6.1.24 guanylyl-3',5'-uridine + H2O GpU Aspergillus oryzae guanosine 3'-phosphate + uridine - ? 373364 4.6.1.24 additional information synthesis of guanylylnucleosides, oligoguanylate, guanosine-containing oligonucleotides with (3'-5')phosphodiester bonds, synthetic reaction can lead to the formation of unnatural (2'-5')-phosphodiester bonds, not: DNA, L-guanosine 2',3'-cyclic phosphate, L-inosine 2',3'-cyclic phosphate Aspergillus oryzae ? - ? 89 4.6.1.24 additional information heteroduplex of ribooligonucleotides are substrates for the enzyme Aspergillus oryzae ? - ? 89 4.6.1.24 additional information endonucleolytic activity is assayed using derivates of BR13, 5'-GGGACAGUAUUUG-3', a model oligonucleotide substrate for studies of RNase E and RNase G Escherichia coli ? - ? 89 4.6.1.24 additional information the backbone dynamics of RNase T1, complexed with the productive substrate exo-cGPS isomer, in comparison to the RNase T1, complexed with the nonproductive substrate 3'GMP, is analyzed Aspergillus oryzae ? - ? 89 4.6.1.24 additional information in vivo binase affects the quantity of proapoptotic and antiapoptotic mRNAs, as expression of the p53 and hSK4 genes is increased and expression of the bcl-2 gene is reduced Bacillus intermedius ? - ? 89 4.6.1.24 additional information barnase catalyses the overall hydrolysis of single-stranded RNA preferentially at guanylyl residues, yielding new guanosine 3'-phosphate and 5'-OH ends, in a two-step process with cleavage of the RNA chain by transesterification of a 5'-phosphoester bond to form a guanosine 2',3'-cyclic phosphate terminus in the first step, followed by its hydrolysis to a 3'-phosphate product in the second independent step Bacillus amyloliquefaciens ? - ? 89 4.6.1.24 additional information binase catalyses the overall hydrolysis of single-stranded RNA preferentially at guanylyl residues, yielding new guanosine 3'-phosphate and 5'-OH ends, in a two-step process with cleavage of the RNA chain by transesterification of a 5'-phosphoester bond to form a guanosine 2',3'-cyclic phosphate terminus in the first step, followed by its hydrolysis to a 3'-phosphate product in the second independent step Bacillus pumilus ? - ? 89 4.6.1.24 additional information assay substrate is commercial yeast RNA, rates of release of GMP and cGMP show base specificity of enzyme mutant D19N/D22N/E25Q/D31N/D38N/E50Q/E57Q/E76Q/D77N/D79N/E92Q/D93N Hericium erinaceus ? - ? 89 4.6.1.24 additional information assay substrate is commercial yeast RNA, the enzyme is a guanylic acid-specific RNase Pleurotus ostreatus ? - ? 89 4.6.1.24 additional information the enzyme cleaves single-stranded RNA by catalyzing the hydrolysis of phosphodiester bonds specifically at the 3'-side of guanosine nucleotides Aspergillus oryzae ? - ? 89 4.6.1.24 additional information the enzyme hydrolyzes specifically the 3'-phosphodiester bond of guanylic acid in RNA, mechanism of interaction of RNase T1 with its substrates, overview. His40 and His92 are deduced to be involved in the active site, overview. Carboxymethylated-RNase T1 possesses almost the same binding ability toward guanosine as intact RNase T1, whereas the binding ability toward 2' or 3'-guanylic acid is considerably lowered by carboxymethylation of Glu58 Aspergillus oryzae ? - ? 89 4.6.1.24 additional information the enzyme is a guanylic acid-specific ribonuclease Pleurotus ostreatus ? - ? 89 4.6.1.24 poly(I) + H2O - Kitasatospora aureofaciens ? - ? 73538 4.6.1.24 polyinosinic acid + H2O polyI Aspergillus oryzae ? - ? 374385 4.6.1.24 RNA + H2O - Aspergillus oryzae Hydrolyzed RNA - ? 12775 4.6.1.24 RNA + H2O - Escherichia coli Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Neurospora crassa Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Aspergillus niger Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Ustilago sphaerogena Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Chalara sp. Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Fusarium verticillioides Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O - Fungi Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O essential requirements: keto group at 6 position, trivalent nitrogen at 7 position of purine base Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O first step (r): cleavage of internucleotide bonds between 3'-guanylic acid groups and the 5'-hydroxyl groups of the adjacent nucleotides with the intermediary formation of guanosine 2',3'-cyclic phosphate, second step (ir): hydrolysis of cyclic phosphate to produce 3'-guanylate Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O specificity Aspergillus oryzae Hydrolyzed RNA 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp ? 12775 4.6.1.24 RNA + H2O two-stage endonucleolytic cleavage to 3'-phosphomononucleotides and 3'-phosphooligonucleotides ending in Gp with 2,3'-cyclic phosphate intermediates Aspergillus oryzae ? - ? 141227 4.6.1.24 RNA + H2O identification of RNase T1 cleavage fragments of Bacillus megaterium ribosomal 5S RNA Aspergillus oryzae ? - ? 141227 4.6.1.24 single-stranded RNA + H2O the enzyme specifically hydrolyzes single-stranded RNA at G sites. RNase T1 does not recognize sites containing 8-oxo-7,8-dihydroguanosine Aspergillus oryzae ? - ? 93110 4.6.1.24 single-stranded RNA + H2O the enzyme specifically hydrolyzes single-stranded RNA at G sites. RNase T1 does not recognize sites containing 8-oxo-7,8-dihydroguanosine Aspergillus oryzae ATCC 42149 ? - ? 93110 4.6.1.24 [RNA] containing guanosine + H2O energetics of ribonuclease catalysis Aspergillus oryzae an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] - ? 450893