4.4.1.15 3-chloro-D-alanine + H2O - Escherichia coli pyruvate + NH3 + Cl- - ? 363501 4.4.1.15 3-chloro-D-alanine + H2O - Pseudomonas putida pyruvate + NH3 + Cl- - ? 363501 4.4.1.15 3-chloro-D-alanine + H2O alpha,beta-elimination, more effective substrate than D-cysteine Escherichia coli pyruvate + NH3 + Cl- - ? 363501 4.4.1.15 3-chloro-D-alanine + H2O - Pseudomonas putida CR 1-1 pyruvate + NH3 + Cl- - ? 363501 4.4.1.15 3-chloro-D-alanine + H2O - Escherichia coli W3110 / ATCC 27325 pyruvate + NH3 + Cl- - ? 363501 4.4.1.15 3-chloro-D-alanine + H2O alpha,beta-elimination, more effective substrate than D-cysteine Escherichia coli W3110 / ATCC 27325 pyruvate + NH3 + Cl- - ? 363501 4.4.1.15 3-chloro-D-alanine + thioglycolic acid i.e. mercaptoacetic acid, beta-replacement reaction Escherichia coli S-carboxymethyl-D-cysteine product is further metabolized to form pyruvate ? 363502 4.4.1.15 3-chloro-D-alanine + thioglycolic acid i.e. mercaptoacetic acid, beta-replacement reaction Escherichia coli W3110 / ATCC 27325 S-carboxymethyl-D-cysteine product is further metabolized to form pyruvate ? 363502 4.4.1.15 D-allocystathionine + H2O - Escherichia coli ? - ? 363511 4.4.1.15 D-cysteine + H2O - Escherichia coli sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Nicotiana tabacum sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Arabidopsis thaliana sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Enterobacter cloacae sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Pseudomonas putida sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Klebsiella pneumoniae sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Citrobacter freundii sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Cucurbita pepo sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Phormidium uncinatum sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Synechococcus elongatus sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Solanum lycopersicum sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Salmonella enterica subsp. enterica serovar Typhimurium sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Methylorubrum extorquens sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - [Chlorella] fusca sulfide + NH3 + pyruvate additional product suggested ? 363500 4.4.1.15 D-cysteine + H2O - Spinacia oleracea sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ? 363500 4.4.1.15 D-cysteine + H2O - [Chlorella] fusca sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ? 363500 4.4.1.15 D-cysteine + H2O alpha,beta-elimination Escherichia coli sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol Spinacia oleracea sulfide + NH3 + pyruvate additional product suggested ? 363500 4.4.1.15 D-cysteine + H2O very specific, no activity with L-cysteine, mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester and dithioerythritol Spinacia oleracea sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ? 363500 4.4.1.15 D-cysteine + H2O very specific, only traces of activity with mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester [Chlorella] fusca sulfide + NH3 + pyruvate additional product suggested ? 363500 4.4.1.15 D-cysteine + H2O very specific, only traces of activity with mercaptoacetic acid, mercaptoethanol, D-cystine, L-cystine, cysteamine, L-cysteine methylester [Chlorella] fusca sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ? 363500 4.4.1.15 D-cysteine + H2O plant sulfur metabolism Nicotiana tabacum sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O plant sulfur metabolism Cucurbita pepo sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O plant sulfur metabolism Spinacia oleracea sulfide + NH3 + pyruvate additional product suggested ? 363500 4.4.1.15 D-cysteine + H2O plant sulfur metabolism Spinacia oleracea sulfide + NH3 + pyruvate more sulfide than pyruvate and ammonium formed ? 363500 4.4.1.15 D-cysteine + H2O physiological function unknown, detoxification of D-cysteine suggested, contributes to utilization of D-cysteine as sulfur source Escherichia coli sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O 37°C, pH 8.0 Arabidopsis thaliana sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O Ser78 and Gln77 are key determinants of enzyme specificity and the phenolate of Tyr287 is responsible for Calpha proton abstraction from D-Cys Salmonella enterica subsp. enterica serovar Typhimurium sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Pseudomonas putida CR 1-1 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Methylorubrum extorquens DSM 1338 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Pseudomonas putida UW4 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Escherichia coli W3110 / ATCC 27325 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O alpha,beta-elimination Escherichia coli W3110 / ATCC 27325 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Synechococcus elongatus PCC 7942 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cysteine + H2O - Phormidium uncinatum CU 1462/7 sulfide + NH3 + pyruvate - ? 363500 4.4.1.15 D-cystine + H2O - Escherichia coli ? - ? 363503 4.4.1.15 DL-lanthionine + H2O - Escherichia coli ? - ? 363512 4.4.1.15 DL-selenocysteine + H2O very poor substrate Escherichia coli selenide + L-alanine - ? 363514 4.4.1.15 DL-selenocystine + H2O - Escherichia coli ? - ? 363515 4.4.1.15 L-cysteine + H2O - Escherichia coli sulfide + NH3 + pyruvate - ? 368481 4.4.1.15 L-cysteine + H2O 5% activity compared to D-cysteine Arabidopsis thaliana sulfide + NH3 + pyruvate - ? 368481 4.4.1.15 L-selenocystine + H2O - Escherichia coli selenide + L-alanine - ? 401287 4.4.1.15 additional information L-cysteine is not utilized Escherichia coli ? - ? 89 4.4.1.15 additional information L-cysteine is not utilized Spinacia oleracea ? - ? 89 4.4.1.15 additional information L-cysteine is not utilized Enterobacter cloacae ? - ? 89 4.4.1.15 additional information L-cysteine is not utilized Klebsiella pneumoniae ? - ? 89 4.4.1.15 additional information L-cysteine is not utilized [Chlorella] fusca ? - ? 89 4.4.1.15 additional information L-cysteine is not utilized Citrobacter freundii ? - ? 89 4.4.1.15 additional information extracellular production of hydrogen selenide accounts for thiol-assisted toxicity of selenite against Saccharomyces cerevisiae Escherichia coli ? - ? 89 4.4.1.15 additional information Site-directed mutagenesis shows that altering only two amino acid residues within the predicted active site served to change the enzyme from D-cysteine desulfhydrase to 1-aminocyclopropane-1-carboxylate deaminase. Solanum lycopersicum ? - ? 89 4.4.1.15 additional information Site-directed mutagenesis shows that altering two amino acid residues at the same positions within the active site of tive site served to change the enzyme from D-cysteine desulfhydrase to deaminase from Pseudomonas putida UW4 the enzyme is converted into D-cysteine desulfhydrase. Pseudomonas putida ? - ? 89 4.4.1.15 additional information the enzyme is also active with beta-chloro-D-alanine which is converted to pyruvate, chloride, and NH3, EC 4.5.1.2. D-Ser is a poor substrate while the enzyme is inactive with respect to L-Ser and 1-amino-1-carboxy cyclopropane, substrate specificity and ligand binding structures, detailed overview. Ser78 and Gln77 are key determinants of enzyme specificity and the phenolate of Tyr287 is responsible for Calpha proton abstraction from D-Cys Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 89 4.4.1.15 additional information no substrate: L-cysteine, 1-aminocyclopropane-1-carboxylate Methylorubrum extorquens ? - ? 89 4.4.1.15 additional information no substrate: L-cysteine, 1-aminocyclopropane-1-carboxylate Methylorubrum extorquens DSM 1338 ? - ? 89 4.4.1.15 additional information Site-directed mutagenesis shows that altering two amino acid residues at the same positions within the active site of tive site served to change the enzyme from D-cysteine desulfhydrase to deaminase from Pseudomonas putida UW4 the enzyme is converted into D-cysteine desulfhydrase. Pseudomonas putida UW4 ? - ? 89 4.4.1.15 additional information L-cysteine is not utilized Escherichia coli W3110 / ATCC 27325 ? - ? 89 4.4.1.15 O-acetyl-D-serine + H2O alpha,beta-elimination reaction Escherichia coli ? - ? 363517 4.4.1.15 O-acetyl-D-serine + H2O alpha,beta-elimination reaction Escherichia coli W3110 / ATCC 27325 ? - ? 363517 4.4.1.15 O-acetyl-D-serine + sulfide beta-replacement reaction Escherichia coli D-cysteine + ? - ? 363516 4.4.1.15 O-acetyl-D-serine + sulfide beta-replacement reaction Escherichia coli W3110 / ATCC 27325 D-cysteine + ? - ? 363516 4.4.1.15 O-methyl-DL-serine + H2O poor substrate Escherichia coli ? - ? 363513 4.4.1.15 S-benzyl-D-cysteine + H2O - Escherichia coli benzyl hydrosulfide + pyruvate + NH3 - ? 363509 4.4.1.15 S-carboxymethyl-D-cysteine + H2O - Escherichia coli mercaptoacetic acid + pyruvate + NH3 - ? 363510 4.4.1.15 S-ethyl-D-cysteine + H2O - Escherichia coli ethanethiol + pyruvate + NH3 - ? 363505 4.4.1.15 S-methyl-D-cysteine + H2O - Escherichia coli methanethiol + pyruvate + NH3 - ? 363504 4.4.1.15 S-n-butyl-D-cysteine + H2O - Escherichia coli butane-1-thiol + pyruvate + NH3 - ? 363507 4.4.1.15 S-n-propyl-D-cysteine + H2O - Escherichia coli propane-1-thiol + pyruvate + NH3 - ? 363506 4.4.1.15 S-phenyl-D-cysteine + H2O - Escherichia coli benzenthiol + pyruvate + NH3 - ? 363508