4.3.1.2 (2E)-2-butylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 59% Clostridium tetanomorphum ? - r 429464 4.3.1.2 (2E)-2-ethylbut-2-enedioate + NH3 wild-type: conversion 74%, mutant L384A: conversion 76% Clostridium tetanomorphum ? - r 429465 4.3.1.2 (2E)-2-hexylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 53% Clostridium tetanomorphum ? - r 429466 4.3.1.2 (2E)-2-methylbut-2-enedioate + NH3 wild-type: conversion 80%, mutant L384A: conversion 75% Clostridium tetanomorphum ? - r 429467 4.3.1.2 (2E)-2-pentylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 52% Clostridium tetanomorphum ? - r 429468 4.3.1.2 (2E)-2-phenylbut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 90% Clostridium tetanomorphum ? - r 429469 4.3.1.2 (2E)-2-propylbut-2-enedioate + NH3 wild-type: conversion 57%, mutant L384A: conversion 67% Clostridium tetanomorphum ? - r 429470 4.3.1.2 (2R,3S)-3-chloroaspartic acid - Citrobacter amalonaticus 2-chloro-2-butenedioic acid - ? 380791 4.3.1.2 (2R,3S)-3-chloroaspartic acid - Citrobacter amalonaticus YG-1002 2-chloro-2-butenedioic acid - ? 380791 4.3.1.2 (2S)-2-hydrazinosuccinic acid - Escherichia coli fumarate + hydrazine - ? 2256 4.3.1.2 (2S)-aspartate - Clostridium tetanomorphum fumarate + NH3 - ? 368450 4.3.1.2 (2S)-N,N-dimethylaspartic acid - Escherichia coli fumarate + dimethylamine - ? 2265 4.3.1.2 (2S)-N-ethylaspartic acid - Escherichia coli fumarate + ethylamine - ? 2264 4.3.1.2 (2S)-N-hydroxyaspartic acid - Escherichia coli fumarate + hydroxylamine - ? 2266 4.3.1.2 (2S)-N-methoxyaspartic acid - Escherichia coli fumarate + methoxylamine - ? 2269 4.3.1.2 (2S)-N-methylaspartic acid - Escherichia coli fumarate + methylamine - ? 2261 4.3.1.2 (2S,3R)-3-ethylaspartic acid - Clostridium tetanomorphum ? - ? 2252 4.3.1.2 (2S,3R)-3-ethylaspartic acid - Citrobacter amalonaticus ? - ? 2252 4.3.1.2 (2S,3R)-3-ethylaspartic acid - Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2252 4.3.1.2 (2S,3S)-3-ethylaspartate - Citrobacter amalonaticus ? - ? 368451 4.3.1.2 (2S,3S)-3-ethylaspartic acid - Bacterium cadaveris ? - ? 2253 4.3.1.2 (2S,3S)-3-ethylaspartic acid - Citrobacter freundii ? - ? 2253 4.3.1.2 (2S,3S)-3-ethylaspartic acid - Clostridium tetanomorphum ? - ? 2253 4.3.1.2 (2S,3S)-3-ethylaspartic acid - Morganella morganii ? - ? 2253 4.3.1.2 (2S,3S)-3-ethylaspartic acid - Citrobacter amalonaticus ? - ? 2253 4.3.1.2 (2S,3S)-3-ethylaspartic acid - Carboxydothermus hydrogenoformans ? - ? 2253 4.3.1.2 (2S,3S)-3-methylaspartate - Citrobacter amalonaticus mesaconate + NH3 - r 380795 4.3.1.2 (2S,3S)-3-methylaspartate - Citrobacter amalonaticus YG-1002 mesaconate + NH3 - r 380795 4.3.1.2 (2S,3S)-N-hydroxy-3-ethylaspartic acid - Escherichia coli ethylfumarate + hydroxylamine - ? 2268 4.3.1.2 (2S,3S)-N-hydroxy-3-methylaspartic acid - Escherichia coli mesaconate + hydroxylamine - ? 2267 4.3.1.2 (2S,3S)-N-methoxy-3-methylaspartic acid - Escherichia coli mesaconate + methoxylamine - ? 2270 4.3.1.2 (2Z)-2-(benzyloxy)but-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 60% Clostridium tetanomorphum ? - r 429488 4.3.1.2 (2Z)-2-(benzylsulfanyl)but-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 42% Clostridium tetanomorphum ? - r 429489 4.3.1.2 (2Z)-2-(ethylsulfanyl)but-2-enedioate + NH3 wild-type: conversion 36%, mutant L384A: conversion 50% Clostridium tetanomorphum ? - r 429490 4.3.1.2 (2Z)-2-(phenylsulfanyl)but-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 34% Clostridium tetanomorphum ? - r 429491 4.3.1.2 (2Z)-2-ethoxybut-2-enedioate + NH3 wild-type: conversion 46%, mutant L384A: conversion 43% Clostridium tetanomorphum ? - r 429492 4.3.1.2 (2Z)-2-phenoxybut-2-enedioate + NH3 wild-type: conversion 0%, mutant L384A: conversion 44% Clostridium tetanomorphum ? - r 429493 4.3.1.2 (S)-aspartate - Fusobacterium varium fumarate + NH3 - ? 368448 4.3.1.2 2-hydrazino-3-chlorosuccinic acid - Escherichia coli chlorofumarate + hydrazine - ? 2257 4.3.1.2 2-hydrazino-3-ethylsuccinic acid - Escherichia coli ethylfumarate + hydrazine - ? 2258 4.3.1.2 2-hydrazino-3-isopropylsuccinic acid - Escherichia coli isopropylfumarate + hydrazine - ? 2259 4.3.1.2 2-hydrazino-3-propylsuccinic acid - Escherichia coli propylfumarate + hydrazine - ? 2260 4.3.1.2 3-bromoaspartate - Bacterium cadaveris bromofumarate + NH3 - ? 2247 4.3.1.2 3-bromoaspartate - Clostridium tetanomorphum bromofumarate + NH3 - ? 2247 4.3.1.2 3-bromoaspartate - Clostridium tetanomorphum bromofumarate + NH3 during reaction, enzyme becomes inactive. This is reduced in the presence of alternative substrates ? 2247 4.3.1.2 3-chloroaspartate - Clostridium tetanomorphum chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter freundii chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Morganella morganii chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter amalonaticus chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Citrobacter amalonaticus YG-1002 chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate - Clostridium tetanomorphum H1 / ATCC 15920 chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate - Clostridium tetanomorphum NCIMB 11547 chlorofumarate + NH3 - ? 2246 4.3.1.2 3-chloroaspartate (2R,3S)-3-chloroaspartic acid Morganella morganii YG-0601 chlorofumarate + NH3 - ? 2246 4.3.1.2 3-fluoroaspartate - Clostridium tetanomorphum fluorofumarate + NH3 not a good substrate ? 2248 4.3.1.2 ? + NH4Cl - Citrobacter freundii ? - ? 2254 4.3.1.2 ? + NH4Cl - Morganella morganii ? - ? 2254 4.3.1.2 beta-ethylaspartate - Bacterium cadaveris ? - ? 2243 4.3.1.2 beta-ethylaspartate - Clostridium tetanomorphum ? - ? 2243 4.3.1.2 beta-ethylaspartate 3-ethylaspartic acid Clostridium tetanomorphum ? - ? 2243 4.3.1.2 beta-ethylaspartate 3-ethylaspartate Clostridium tetanomorphum ? - ? 2243 4.3.1.2 beta-ethylaspartate - Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2243 4.3.1.2 beta-ethylaspartate 3-ethylaspartic acid Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2243 4.3.1.2 beta-isopropylaspartate - Clostridium tetanomorphum ? - ? 2245 4.3.1.2 beta-propylaspartate - Clostridium tetanomorphum ? - ? 2244 4.3.1.2 beta-propylaspartate beta-n-propylaspartate Clostridium tetanomorphum ? - ? 2244 4.3.1.2 erythro-3-methylaspartate - Bacterium cadaveris ? - ? 2242 4.3.1.2 erythro-3-methylaspartate - Clostridium tetanomorphum ? - ? 2242 4.3.1.2 erythro-3-methylaspartate erythro-beta-methyl-L-aspartate Clostridium tetanomorphum ? - ? 2242 4.3.1.2 erythro-3-methylaspartate (2S,3R)-3-methylaspartic acid Escherichia coli ? - ? 2242 4.3.1.2 erythro-3-methylaspartate (2S,3R)-3-methylaspartic acid Bacterium cadaveris ? - ? 2242 4.3.1.2 erythro-3-methylaspartate (2S,3R)-3-methylaspartic acid Clostridium tetanomorphum ? - ? 2242 4.3.1.2 erythro-3-methylaspartate erythro-3-methyl-L-aspartate Clostridium tetanomorphum ? - ? 2242 4.3.1.2 erythro-3-methylaspartate erythro-beta-methylaspartate Clostridium tetanomorphum ? - ? 2242 4.3.1.2 erythro-3-methylaspartate - Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2242 4.3.1.2 erythro-3-methylaspartate erythro-3-methyl-L-aspartate Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2242 4.3.1.2 erythro-3-methylaspartate erythro-beta-methylaspartate Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2242 4.3.1.2 ethylfumarate + NH3 - Citrobacter freundii ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Clostridium tetanomorphum ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Morganella morganii ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Citrobacter amalonaticus ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Carboxydothermus hydrogenoformans ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Citrobacter amalonaticus YG-1002 ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Clostridium tetanomorphum NCIMB 11547 ? - ? 2249 4.3.1.2 ethylfumarate + NH3 - Morganella morganii YG-0601 ? - ? 2249 4.3.1.2 fumarate + NH3 - Carboxydothermus hydrogenoformans ? - ? 430186 4.3.1.2 hydrazino-3-methylsuccinic acid - Escherichia coli mesaconate + hydrazine - ? 2255 4.3.1.2 iso-propylfumarate + NH3 - Clostridium tetanomorphum ? - ? 2250 4.3.1.2 L-aspartate - Clostridium tetanomorphum fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate rate of deamination 100 times slower than that of L-threo-3-methylaspartate Clostridium tetanomorphum fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate (2S,3R) [3-3H]aspartic acid Bacterium cadaveris fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate (2S,3R) [3-3H]aspartic acid Clostridium tetanomorphum fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate (2S,3R) [3-3H]aspartic acid Clostridium tetanomorphum fumarate + NH3 amination 1.8 times faster than the amination of mesaconate ? 2188 4.3.1.2 L-aspartate (2S)-aspartate Clostridium tetanomorphum fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate (2S)-aspartate Clostridium tetanomorphum fumarate + NH3 amination 1.8 times faster than the amination of mesaconate ? 2188 4.3.1.2 L-aspartate (2S)-aspartate Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate - Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate rate of deamination 100 times slower than that of L-threo-3-methylaspartate Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3 - ? 2188 4.3.1.2 L-aspartate (2S,3R) [3-3H]aspartic acid Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3 amination 1.8 times faster than the amination of mesaconate ? 2188 4.3.1.2 L-aspartate (2S)-aspartate Clostridium tetanomorphum H1 / ATCC 15920 fumarate + NH3 amination 1.8 times faster than the amination of mesaconate ? 2188 4.3.1.2 L-erythro-3-methylaspartate i.e. (2S,3R)-3-methylaspartate, slow syn elimination Clostridium tetanomorphum mesaconate + NH3 - ? 368449 4.3.1.2 L-erythro-3-methylaspartate i.e. (2S,3R)-3-methylaspartate, slow syn elimination Clostridium tetanomorphum mesaconate + NH3 - r 368449 4.3.1.2 L-threo-3-methylaspartate - Clostridium tetanomorphum mesaconate + NH3 - r 2240 4.3.1.2 L-threo-3-methylaspartate - Citrobacter amalonaticus mesaconate + NH3 - r 2240 4.3.1.2 L-threo-3-methylaspartate - Fusobacterium varium mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate - Fusobacterium varium mesaconate + NH3 - r 2240 4.3.1.2 L-threo-3-methylaspartate r Escherichia coli mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Bacterium cadaveris mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Morganella morganii mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Raoultella planticola mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter koseri mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate threo-beta-methyl-L-aspartate Clostridium tetanomorphum mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate beta-methylaspartate Clostridium tetanomorphum mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Escherichia coli mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Raoultella planticola mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter koseri mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate i.e. (2S,3S)-3-methylaspartate Clostridium tetanomorphum mesaconate + NH3 - r 2240 4.3.1.2 L-threo-3-methylaspartate the enzyme is part of a catabolic pathway for L-glutamate Clostridium tetanomorphum mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus YG-1002 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus YG-1002 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus YG1002 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus YG1002 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii YG-0504 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii YG-0504 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Morganella morganii NCIMB 232 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii NCIMB 232 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Raoultella planticola IAM 1133 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Raoultella planticola IAM 1133 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii TPU 0011 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii TPU 0011 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate - Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3 - r 2240 4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate i.e. (2S,3S)-3-methylaspartate Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3 - r 2240 4.3.1.2 L-threo-3-methylaspartate threo-beta-methyl-L-aspartate Clostridium tetanomorphum H1 / ATCC 15920 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter koseri JCM 1659 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter koseri JCM 1659 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus TPU 6323 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum NCIMB 11547 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Morganella morganii NCIMB 9525 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii NCIMB 9525 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Citrobacter koseri JCM 1658 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter koseri JCM 1658 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Morganella morganii YG-0601 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii YG-0601 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate r Morganella morganii NCIMB 10466 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii NCIMB 10466 mesaconate + NH3 - ? 2240 4.3.1.2 L-threo-3-methylaspartate - Escherichia coli ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Bacterium cadaveris ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter freundii ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Clostridium tetanomorphum ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Morganella morganii ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Raoultella planticola ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter koseri ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter amalonaticus ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter amalonaticus YG-1002 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter amalonaticus YG1002 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter freundii YG-0504 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Morganella morganii NCIMB 232 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Raoultella planticola IAM 1133 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter freundii TPU 0011 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter koseri JCM 1659 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter amalonaticus TPU 6323 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Clostridium tetanomorphum NCIMB 11547 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Morganella morganii NCIMB 9525 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Citrobacter koseri JCM 1658 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Morganella morganii YG-0601 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartate - Morganella morganii NCIMB 10466 ? - ? 369478 4.3.1.2 L-threo-3-methylaspartic acid assay at pH 9.0, 30°C Clostridium tetanomorphum mesaconate + NH3 - ? 409723 4.3.1.2 mesaconate + (aminooxy)ethane mutant Q73A, conversion: 100%, yield: 61% Clostridium tetanomorphum ? - r 430368 4.3.1.2 mesaconate + (aminooxy)methane mutant Q73A, conversion: 99%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430369 4.3.1.2 mesaconate + 1-cyclopropylmethanamine mutant Q73A, conversion: 61%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430370 4.3.1.2 mesaconate + 1-cyclopropylmethanamine mutant Q73A, conversion: 96%, yield: 28%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430370 4.3.1.2 mesaconate + 2-aminoethanol mutant Q73A, conversion: 37%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430371 4.3.1.2 mesaconate + 2-methoxyethanamine mutant Q73A, conversion: 100%, yield: 53% Clostridium tetanomorphum ? - r 430372 4.3.1.2 mesaconate + 2-methoxyethanamine mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430372 4.3.1.2 mesaconate + 3-aminopropan-1-ol mutant Q73A, conversion: 56%, excess of threo isomer over erythro isomer: 80% Clostridium tetanomorphum ? - r 430373 4.3.1.2 mesaconate + 3-aminopropan-1-ol mutant Q73A, conversion: 99%, yield: 57% Clostridium tetanomorphum ? - r 430373 4.3.1.2 mesaconate + aminobutane mutant Q73A, conversion: 60%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430375 4.3.1.2 mesaconate + aminobutane mutant Q73A, conversion: 97%, yield: 33%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430375 4.3.1.2 mesaconate + aniline mutant Q73A, conversion: 6%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430374 4.3.1.2 mesaconate + cyclobutanamine mutant Q73A, conversion: 65%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430376 4.3.1.2 mesaconate + cyclobutanamine mutant Q73A, conversion: 99%, yield: 74%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430376 4.3.1.2 mesaconate + cyclohexanamine mutant Q73A, conversion: 21%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430377 4.3.1.2 mesaconate + cyclopentanamine mutant Q73A, conversion: 36%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430378 4.3.1.2 mesaconate + cyclopropanamine mutant Q73A, conversion: 100%, yield: 48% Clostridium tetanomorphum ? - r 430379 4.3.1.2 mesaconate + cyclopropanamine mutant Q73A, conversion: 75%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430379 4.3.1.2 mesaconate + ethanamine mutant Q73A, conversion: 100%, yield: 75%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430380 4.3.1.2 mesaconate + ethanamine mutant Q73A, conversion: 51%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430380 4.3.1.2 mesaconate + ethane-1,2-diamine mutant Q73A, conversion: 90%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430381 4.3.1.2 mesaconate + ethane-1,2-diamine mutant Q73A, conversion: 99%, yield: 67% Clostridium tetanomorphum ? - r 430381 4.3.1.2 mesaconate + ethylamine - Carboxydothermus hydrogenoformans ? - ? 430382 4.3.1.2 mesaconate + hexanamine mutant Q73A, conversion: 17%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430383 4.3.1.2 mesaconate + hydrazine - Carboxydothermus hydrogenoformans ? - ? 430384 4.3.1.2 mesaconate + hydroxylamine - Carboxydothermus hydrogenoformans ? - ? 430385 4.3.1.2 mesaconate + methanamine mutant Q73A, conversion: 100%, yield: 96%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430386 4.3.1.2 mesaconate + methanamine mutant Q73A, conversion: 74%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430386 4.3.1.2 mesaconate + methoxylamine - Carboxydothermus hydrogenoformans ? - ? 430387 4.3.1.2 mesaconate + methylamine - Carboxydothermus hydrogenoformans ? - ? 430388 4.3.1.2 mesaconate + N-methylethane-1,2-diamine mutant Q73A, conversion: 80%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430389 4.3.1.2 mesaconate + NH3 - Carboxydothermus hydrogenoformans ? - ? 430390 4.3.1.2 mesaconate + NH3 - Clostridium tetanomorphum L-threo-3-methylaspartate - r 430391 4.3.1.2 mesaconate + NH3 mutant Q73A, conversion: 100%, yield: 79%, enantiomeric excess: more than 99% Clostridium tetanomorphum L-threo-3-methylaspartate - r 430391 4.3.1.2 mesaconate + NH3 mutant Q73A, conversion: 79%, excess of threo isomer over erythro isomer: 5% Clostridium tetanomorphum L-threo-3-methylaspartate - r 430391 4.3.1.2 mesaconate + NH3 wild-type: conversion 100%, mutant L384A: conversion 99% Clostridium tetanomorphum L-threo-3-methylaspartate - r 430391 4.3.1.2 mesaconate + NH3 conversion 2h: 73% (wild-type), diastereomeric product ratio (threo:erythro) after 2h: 89:11 Clostridium tetanomorphum L-threo-3-methylaspartate + L-erythro-3-methylaspartate - r 430392 4.3.1.2 mesaconate + NH4Cl - Escherichia coli methylaspartate + HCl - r 392580 4.3.1.2 mesaconate + pentanamine mutant Q73A, conversion: 29%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430393 4.3.1.2 mesaconate + pentanamine mutant Q73A, conversion: 91%, yield: 14%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430393 4.3.1.2 mesaconate + propan-2-amine mutant Q73A, conversion: 8%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430394 4.3.1.2 mesaconate + propanamine mutant Q73A, conversion: 53%, excess of threo isomer over erythro isomer: more than 95%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430395 4.3.1.2 mesaconate + propanamine mutant Q73A, conversion: 99%, yield: 53%, enantiomeric excess: more than 99% Clostridium tetanomorphum ? - r 430395 4.3.1.2 mesaconate + propane-1,3-diamine mutant Q73A, conversion: 100%, yield: 61% Clostridium tetanomorphum ? - r 430396 4.3.1.2 mesaconate + propane-1,3-diamine mutant Q73A, conversion: 78%, excess of threo isomer over erythro isomer: more than 95% Clostridium tetanomorphum ? - r 430396 4.3.1.2 mesaconic acid + NH3 assay at pH 9.0, 30°C Clostridium tetanomorphum L-threo-3-methylaspartate - r 409860 4.3.1.2 methylfumarate + NH3 - Carboxydothermus hydrogenoformans ? - ? 430418 4.3.1.2 additional information - Escherichia coli ? - ? 89 4.3.1.2 additional information - Bacterium cadaveris ? - ? 89 4.3.1.2 additional information - Citrobacter freundii ? - ? 89 4.3.1.2 additional information - Clostridium tetanomorphum ? - ? 89 4.3.1.2 additional information - Morganella morganii ? - ? 89 4.3.1.2 additional information - Citrobacter amalonaticus ? - ? 89 4.3.1.2 additional information enzyme is specific for the L-configuration Clostridium tetanomorphum ? - ? 89 4.3.1.2 additional information catalytic rates for the enzymatic amination of fumarate and mesaconate are faster than the rates of the corresponding deamination reactions Clostridium tetanomorphum ? - ? 89 4.3.1.2 additional information enzyme is involved in the catabolic pathway for glutamate Clostridium tetanomorphum ? - ? 89 4.3.1.2 additional information enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes Clostridium tetanomorphum ? - ? 89 4.3.1.2 additional information ammonia is the natural nucleophile for the enzyme Escherichia coli ? - ? 89 4.3.1.2 additional information no reaction with aspartic acid Citrobacter amalonaticus ? - ? 89 4.3.1.2 additional information - Citrobacter amalonaticus YG-1002 ? - ? 89 4.3.1.2 additional information no reaction with aspartic acid Citrobacter amalonaticus YG-1002 ? - ? 89 4.3.1.2 additional information enzyme is involved in the catabolic pathway for glutamate Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 89 4.3.1.2 additional information - Clostridium tetanomorphum H1 / ATCC 15920 ? - ? 89 4.3.1.2 additional information - Clostridium tetanomorphum NCIMB 11547 ? - ? 89 4.3.1.2 additional information enzyme is associated with an anaerobic degradation of the (S)-glutamic acid in obligat anaerobes Clostridium tetanomorphum NCIMB 11547 ? - ? 89 4.3.1.2 additional information - Morganella morganii YG-0601 ? - ? 89 4.3.1.2 N,3-dimethylaspartic acid - Escherichia coli methylfumarate + methylamine - ? 2262 4.3.1.2 N-methyl-3-ethylaspartic acid - Escherichia coli ethylfumarate + methylamine - ? 2263 4.3.1.2 NH3 + (2E)-2-methylbut-2-enedioate - Fusobacterium varium (2S,3S)-3-methylaspartate major product r 368446 4.3.1.2 NH3 + (2E)-2-methylbut-2-enedioate - Fusobacterium varium (2S,3R)-3-methylaspartate minor product r 368447 4.3.1.2 propylfumarate + NH3 - Carboxydothermus hydrogenoformans ? - ? 430653