4.2.1.9 (+)-2,3-dihydroxy-3-ethylpentanoate 20% of the activity with 2,3-dihydroxyisovalerate Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 18758 4.2.1.9 (2R,3R)-2,3-dihydroxy-3-methylpentanoate - Spinacia oleracea 3-methyl-2-oxopentanoate + H2O - ? 453486 4.2.1.9 (2R,3S)-2,3-dihydroxy-3-methylpentanoate - Spinacia oleracea 3-methyl-2-oxopentanoate + H2O - ? 453487 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Paracoccus denitrificans 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Escherichia coli 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Saccharomyces cerevisiae 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Neurospora crassa 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Cereibacter sphaeroides 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Spinacia oleracea 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Klebsiella aerogenes 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Cupriavidus necator 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Acinetobacter sp. 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Rhodospirillum rubrum 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Chromatium sp. 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Acidovorax facilis 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Achromobacter ruhlandii 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Methanococcus aeolicus 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Methanococcus maripaludis 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Methanococcus voltae 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate no activity with 2S-2,3-dihydroxy-3-methylbutanoate Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate 2R-2,3-dihydroxy-3-methylbutanoate Salmonella enterica subsp. enterica serovar Typhimurium 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Chromatium sp. D 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Acinetobacter sp. 15150 2-oxoisovalerate + H2O - ? 18756 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Saccharolobus solfataricus 3-methyl-2-oxobutanoate + H2O - ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Hevea brasiliensis 3-methyl-2-oxobutanoate + H2O - ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Corynebacterium glutamicum 3-methyl-2-oxobutanoate + H2O i.e. 2-ketoisovalerate ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Corynebacterium glutamicum 3-methyl-2-oxobutanoate + H2O i.e. alpha-ketoisovalerate ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate biosynthesis pathway of the branched chain amino acids valine and isoleucine Saccharolobus solfataricus 3-methyl-2-oxobutanoate + H2O - ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate the enzyme is implicated in latex metabolism Hevea brasiliensis 3-methyl-2-oxobutanoate + H2O - ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Saccharolobus solfataricus P2 3-methyl-2-oxobutanoate + H2O - ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate biosynthesis pathway of the branched chain amino acids valine and isoleucine Saccharolobus solfataricus P2 3-methyl-2-oxobutanoate + H2O - ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Corynebacterium glutamicum ATCC 13032 3-methyl-2-oxobutanoate + H2O i.e. 2-ketoisovalerate ? 415549 4.2.1.9 2,3-dihydroxy-3-methylbutanoate - Corynebacterium glutamicum ATCC 13032 3-methyl-2-oxobutanoate + H2O i.e. alpha-ketoisovalerate ? 415549 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Salmonella enterica subsp. enterica serovar Typhimurium 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Paracoccus denitrificans 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Escherichia coli 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Saccharomyces cerevisiae 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Neurospora crassa 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Cereibacter sphaeroides 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Spinacia oleracea 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Klebsiella aerogenes 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Cupriavidus necator 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Acinetobacter sp. 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Rhodospirillum rubrum 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Chromatium sp. 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Acidovorax facilis 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Achromobacter ruhlandii 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Methanococcus aeolicus 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Methanococcus maripaludis 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Methanococcus voltae 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate 2R,3R-(2,3)-dihydroxy-3-methylpentanoate, at 45% of the activity with 2,3-dihydroxyisovalerate Salmonella enterica subsp. enterica serovar Typhimurium 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate 2R,3S-2,3-dihydroxy-3-methylpentanoate, 2R,3R-2,3-dihydroxy-3-methylpentanoate Spinacia oleracea 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate 2R,3R-dihydroxy-3-methylpentanoate, 2R,3S-dihydroxy-3-methylpentanoate, no activity with isomers with 2S configuration Salmonella enterica subsp. enterica serovar Typhimurium 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Chromatium sp. D 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Acinetobacter sp. 15150 3-methyl-2-oxopentanoate + H2O - ? 18755 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Escherichia coli ? - ? 369584 4.2.1.9 2,3-dihydroxy-3-methylpentanoate - Neurospora crassa ? - ? 369584 4.2.1.9 2,3-dihydroxy-3-methylpentanoate involved in synthesis of Ile Spinacia oleracea ? - ? 369584 4.2.1.9 2,3-dihydroxy-3-methylpentanoate 2R,3R-(2,3)-dihydroxy-3-methylpentanoate Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 369584 4.2.1.9 2,3-dihydroxy-3-methylvalerate - Corynebacterium glutamicum 3-methyl-2-oxovalerate + H2O - ? 402318 4.2.1.9 2,3-dihydroxy-isovalerate - Corynebacterium glutamicum 2-oxoisovalerate - ? 402319 4.2.1.9 2,3-dihydroxybutanoate - Spinacia oleracea ? - ? 18761 4.2.1.9 2,3-dihydroxyisovalerate - Escherichia coli ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate - Neurospora crassa ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate - Methanococcus aeolicus ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate - Methanococcus maripaludis ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate - Methanococcus voltae ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate - Saccharolobus solfataricus ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate involved in biosynthesis of Val Spinacia oleracea ? - ? 369585 4.2.1.9 2,3-dihydroxyisovalerate IlvD, an iron-sulfur enzyme, catalyses the conversion from 2,3-dihydroxyisovalerate to 2-keto-isovalerate and is essential for the branchend-chain amino acid biosynthesis Escherichia coli 2-oxovalerate + H2O - ? 402320 4.2.1.9 2,3-dihydroxyisovalerate - Cupriavidus necator 2-oxoisovalerate + H2O - ? 444613 4.2.1.9 3-cyclopropyl-2,3-dihydroxybutanoate - Spinacia oleracea ? - ? 18762 4.2.1.9 D,L-2,3-dihydroxy-isovalerate - Escherichia coli ? - ? 404757 4.2.1.9 D-arabonate - Saccharolobus solfataricus ? - ? 1981 4.2.1.9 D-arabonate - Saccharolobus solfataricus P2 ? - ? 1981 4.2.1.9 D-erythronate - Spinacia oleracea ? - ? 18760 4.2.1.9 D-fuconate - Saccharolobus solfataricus ? - ? 1977 4.2.1.9 D-fuconate - Saccharolobus solfataricus P2 ? - ? 1977 4.2.1.9 D-Galacturonate - Saccharolobus solfataricus ? - ? 369282 4.2.1.9 D-gluconate - Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O - ? 363554 4.2.1.9 D-gluconate - Saccharolobus solfataricus P2 2-dehydro-3-deoxy-D-gluconate + H2O - ? 363554 4.2.1.9 D-gluconate - Saccharolobus solfataricus 2-keto-3-deoxygluconate + H2O - ? 391743 4.2.1.9 D-Glucuronate - Saccharolobus solfataricus ? - ? 369281 4.2.1.9 D-Glucuronate - Saccharolobus solfataricus P2 ? - ? 369281 4.2.1.9 D-glycerate the enzyme catalyzes the non-natural dehydration of D-glycerate to pyruvate Saccharolobus solfataricus pyruvate + H2O - ? 431760 4.2.1.9 D-glycerate the enzyme catalyzes the non-natural dehydration of D-glycerate to pyruvate Saccharolobus solfataricus P2 pyruvate + H2O - ? 431760 4.2.1.9 D-xylonate - Saccharolobus solfataricus ? - ? 1976 4.2.1.9 D-xylonate - Saccharolobus solfataricus P2 ? - ? 1976 4.2.1.9 DL-2,3-dihydroxyisovalerate - Corynebacterium glutamicum 2-oxo-isovalerate + H2O - ? 415620 4.2.1.9 DL-2,3-dihydroxyisovalerate - Mycobacterium tuberculosis ? - ? 416932 4.2.1.9 DL-2,3-dihydroxyisovalerate - Mycobacterium tuberculosis H37Rv ? - ? 416932 4.2.1.9 L-threonate - Spinacia oleracea ? - ? 18759 4.2.1.9 L-threonate - Aspergillus fumigatus ? - ? 18759 4.2.1.9 additional information third enzyme in the branched-chain amino acid biosynthetic pathway Spinacia oleracea ? - ? 89 4.2.1.9 additional information dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids Saccharolobus solfataricus ? - ? 89 4.2.1.9 additional information reaction product determination by formation of 2,4-dinitrophenylhydrazone from the keto acid product resulting in 2-ketoisovalerate-dinitrophenylhydrazone Corynebacterium glutamicum ? - ? 89 4.2.1.9 additional information dihydroxy-acid dehydratase is one of the key enzymes involved in the biosynthetic pathway of the branched chain amino acids Saccharolobus solfataricus P2 ? - ? 89 4.2.1.9 additional information reaction product determination by formation of 2,4-dinitrophenylhydrazone from the keto acid product resulting in 2-ketoisovalerate-dinitrophenylhydrazone Corynebacterium glutamicum ATCC 13032 ? - ? 89 4.2.1.9 threo-2,3-dihydroxybutanoate at 45% of the activity with 2,3-dihydroxyisovalerate Salmonella enterica subsp. enterica serovar Typhimurium ? - ? 18757