3.6.1.10 ATP + H2O only in presence of Co2+, not with Mg2+, reaction of EC 3.6.1.1 Saccharomyces cerevisiae ADP + phosphate - ? 92967 3.6.1.10 ATP + H2O only in presence of Co2+, not with Mg2+, reaction of EC 3.6.1.1 Saccharomyces cerevisiae ATCC 204508 ADP + phosphate - ? 92967 3.6.1.10 cAMP + H2O - Saccharomyces cerevisiae adenosine + phosphate - ? 455431 3.6.1.10 dATP + H2O - Saccharomyces cerevisiae dADP + phosphate - ? 260008 3.6.1.10 dATP + H2O - Saccharomyces cerevisiae ATCC 204508 dADP + phosphate - ? 260008 3.6.1.10 diphosphate + H2O only in presence of Co2+, not with Mg2+, reaction of EC 3.6.1.1 Saccharomyces cerevisiae 2 phosphate - ? 73540 3.6.1.10 diphosphate + H2O only in presence of Co2+, not with Mg2+, reaction of EC 3.6.1.1 Saccharomyces cerevisiae ATCC 204508 2 phosphate - ? 73540 3.6.1.10 GTP + H2O - Saccharomyces cerevisiae GDP + phosphate - ? 73741 3.6.1.10 GTP + H2O - Saccharomyces cerevisiae ATCC 204508 GDP + phosphate - ? 73741 3.6.1.10 guanosine tetraphosphate + H2O - Saccharomyces cerevisiae guanosine triphosphate + phosphate - ? 455754 3.6.1.10 additional information inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 Saccharomyces cerevisiae ? - ? 89 3.6.1.10 additional information null mutants in Ppn1 accumulate long-chain polyphosphates and are defective in growth in minimal media Saccharomyces cerevisiae ? - ? 89 3.6.1.10 additional information the pathway of particular polyphosphates in yeast is different with endo- and exopolyphosphatase playing critical roles Saccharomyces cerevisiae ? - ? 89 3.6.1.10 additional information enzyme is a bifunctional exo- and endopolyphosphatase, activities of ECs 3.6.1.11 and 3.6.1.10, respectively. No activity with diphosphate, ATP and 4-nitrophenyl phosphate Saccharomyces cerevisiae ? - ? 89 3.6.1.10 additional information DPP1 has endopolyphosphatase activity (EC 3.6.1.10), and low exopolyphosphatase activity (EC 3.6.1.11). Exopolyphosphatases (polyphosphate phosphohydrolases) cleave phosphate from the end of the polyphosphate chain, whereas endopolyphosphatases (polyphosphate depolymerases) split long polyphosphate molecules into oligopolyphosphates Saccharomyces cerevisiae ? - - 89 3.6.1.10 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ? - - 89 3.6.1.10 additional information Ppn1 has endopolyphosphatase activity (EC 3.6.1.10), and high exopolyphosphatase activity (EC 3.6.1.11). The Ppn1 activity with guanosine tetraphosphate is nearly 80% of activity with long-chain polyphosphates. Ppn1 hydrolyzes dATP. Exopolyphosphatases (polyphosphate phosphohydrolases) cleave phosphate from the end of the polyphosphate chain, whereas endopolyphosphatases (polyphosphate depolymerases) split long polyphosphate molecules into oligopolyphosphates Saccharomyces cerevisiae ? - - 89 3.6.1.10 additional information Ppn2 has endopolyphosphatase activity (EC 3.6.1.10), and low exopolyphosphatase activity (EC 3.6.1.11). Exopolyphosphatases (polyphosphate phosphohydrolases) cleave phosphate from the end of the polyphosphate chain, whereas endopolyphosphatases (polyphosphate depolymerases) split long polyphosphate molecules into oligopolyphosphates Saccharomyces cerevisiae ? - - 89 3.6.1.10 additional information TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyP of various chain sizes. The enzyme has a higher affinity for polyP60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay Trypanosoma brucei ? - - 89 3.6.1.10 additional information Ppn2 has endopolyphosphatase activity (EC 3.6.1.10), and low exopolyphosphatase activity (EC 3.6.1.11). Exopolyphosphatases (polyphosphate phosphohydrolases) cleave phosphate from the end of the polyphosphate chain, whereas endopolyphosphatases (polyphosphate depolymerases) split long polyphosphate molecules into oligopolyphosphates Saccharomyces cerevisiae ATCC 204508 ? - - 89 3.6.1.10 additional information Ppn1 has endopolyphosphatase activity (EC 3.6.1.10), and high exopolyphosphatase activity (EC 3.6.1.11). The Ppn1 activity with guanosine tetraphosphate is nearly 80% of activity with long-chain polyphosphates. Ppn1 hydrolyzes dATP. Exopolyphosphatases (polyphosphate phosphohydrolases) cleave phosphate from the end of the polyphosphate chain, whereas endopolyphosphatases (polyphosphate depolymerases) split long polyphosphate molecules into oligopolyphosphates Saccharomyces cerevisiae ATCC 204508 ? - - 89 3.6.1.10 additional information DPP1 has endopolyphosphatase activity (EC 3.6.1.10), and low exopolyphosphatase activity (EC 3.6.1.11). Exopolyphosphatases (polyphosphate phosphohydrolases) cleave phosphate from the end of the polyphosphate chain, whereas endopolyphosphatases (polyphosphate depolymerases) split long polyphosphate molecules into oligopolyphosphates Saccharomyces cerevisiae ATCC 204508 ? - - 89 3.6.1.10 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ATCC 204508 ? - - 89 3.6.1.10 additional information TbNH4 is an endo- and exopolyphosphatase that has similar activity on polyP of various chain sizes. The enzyme has a higher affinity for polyP60 than for polyphosphate700. No activity with 5-diphosphoinositol pentakisphosphate (5-IP7). Malachite green assay Trypanosoma brucei 927/4 GUTat10.1 ? - - 89 3.6.1.10 polyP750 + H2O - Saccharomyces cerevisiae polyP749 + phosphate - ? 453400 3.6.1.10 polyphosphate + H2O - Drosophila melanogaster oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Rattus norvegicus oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Saccharomyces cerevisiae oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Neurospora crassa oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Giardia intestinalis oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Dictyostelium discoideum oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Sulfolobus acidocaldarius oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Caenorhabditis elegans oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Chlamydomonas sp. oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Synechococcus sp. oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Phaeodactylum tricornutum oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Funneliformis mosseae oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Pyrococcus islandicum oligophosphate - ? 141193 3.6.1.10 polyphosphate + H2O - Saccharomyces cerevisiae oligophosphate distributive action on polyP750 produces shorter chains to a limit of about polyP60 as well as the more abundant release of polyP3, limit digestion product is polyP3 ? 141193 3.6.1.10 polyphosphate + H2O polymetaphosphate Aspergillus niger oligophosphate break down stops when the scission products are tetraphosphate or pentaphosphate ? 141193 3.6.1.10 polyphosphate + H2O - Neurospora crassa 28610 oligophosphate - ? 141193 3.6.1.10 polyphosphate 208 + H2O - Saccharomyces cerevisiae oligophosphate the average polyphosphate chain lengths decrease from about 208 to about 10 phosphate residues ? 415136 3.6.1.10 polyphosphate 208 + H2O - Saccharomyces cerevisiae polyphosphate 193 + polyphosphate 15 - ? 421211 3.6.1.10 polyphosphate15 + H2O - Saccharomyces cerevisiae ? - ? 456278 3.6.1.10 polyphosphate15 + H2O - Saccharomyces cerevisiae ATCC 204508 ? - ? 456278 3.6.1.10 polyphosphate208 + H2O - Saccharomyces cerevisiae ? - ? 456279 3.6.1.10 polyphosphate208 + H2O - Saccharomyces cerevisiae ATCC 204508 ? - ? 456279 3.6.1.10 polyphosphate3 + H2O - Saccharomyces cerevisiae ? - ? 456281 3.6.1.10 polyphosphate3 + H2O - Saccharomyces cerevisiae ATCC 204508 ? - ? 456281 3.6.1.10 polyphosphate3 + H2O - Saccharomyces cerevisiae diphosphate + phosphate - ? 456282 3.6.1.10 polyphosphate60 + H2O - Trypanosoma brucei ? - ? 454464 3.6.1.10 polyphosphate60 + H2O - Trypanosoma brucei 927/4 GUTat10.1 ? - ? 454464 3.6.1.10 polyphosphate700 + H2O progression via intermediate chain length polyphosphates Saccharomyces cerevisiae phosphate + triphosphate product analysis ? 385572 3.6.1.10 polyphosphate700 + H2O - Trypanosoma brucei ? - ? 454466 3.6.1.10 polyphosphate700 + H2O - Trypanosoma brucei 927/4 GUTat10.1 ? - ? 454466